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Q31XV2

- HCAE_SHIBS

UniProt

Q31XV2 - HCAE_SHIBS

Protein

3-phenylpropionate/cinnamic acid dioxygenase subunit alpha

Gene

hcaE

Organism
Shigella boydii serotype 4 (strain Sb227)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.UniRule annotation

    Catalytic activityi

    3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+.UniRule annotation
    (2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.UniRule annotation

    Cofactori

    Binds 1 iron ion.UniRule annotation
    Binds 1 2Fe-2S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi85 – 851Iron-sulfur (2Fe-2S)UniRule annotation
    Metal bindingi87 – 871Iron-sulfur (2Fe-2S); via pros nitrogenUniRule annotation
    Metal bindingi105 – 1051Iron-sulfur (2Fe-2S)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur (2Fe-2S); via pros nitrogenUniRule annotation
    Metal bindingi213 – 2131IronUniRule annotation
    Metal bindingi218 – 2181IronUniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 3-phenylpropionate dioxygenase activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciSBOY300268:GJFL-2559-MONOMER.
    UniPathwayiUPA00714.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-phenylpropionate/cinnamic acid dioxygenase subunit alphaUniRule annotation (EC:1.14.12.19UniRule annotation)
    Alternative name(s):
    Digoxigenin subunit alphaUniRule annotation
    Gene namesi
    Name:hcaEUniRule annotation
    Ordered Locus Names:SBO_2562
    OrganismiShigella boydii serotype 4 (strain Sb227)
    Taxonomic identifieri300268 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000007067: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4534533-phenylpropionate/cinnamic acid dioxygenase subunit alphaPRO_0000333706Add
    BLAST

    Interactioni

    Subunit structurei

    This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).UniRule annotation

    Protein-protein interaction databases

    STRINGi300268.SBO_2562.

    Structurei

    3D structure databases

    ProteinModelPortaliQ31XV2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 14299RieskeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.UniRule annotation
    Contains 1 Rieske domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG4638.
    HOGENOMiHOG000105925.
    KOiK05708.
    OMAiKAEWMMH.
    OrthoDBiEOG6W19DT.

    Family and domain databases

    Gene3Di2.102.10.10. 1 hit.
    3.90.380.10. 1 hit.
    HAMAPiMF_01648. HcaE.
    InterProiIPR020875. HcaE.
    IPR017941. Rieske_2Fe-2S.
    IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
    IPR015879. Ring_hydroxy_dOase_asu_C_dom.
    IPR001663. Rng_hydr_dOase-A.
    [Graphical view]
    PfamiPF00355. Rieske. 1 hit.
    PF00848. Ring_hydroxyl_A. 1 hit.
    [Graphical view]
    PRINTSiPR00090. RNGDIOXGNASE.
    SUPFAMiSSF50022. SSF50022. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    PS00570. RING_HYDROXYL_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q31XV2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES    50
    QIPKPGDFFN TYMGEDAVVV VRQKDGSIKA FLNQCRHRAM RVSYADCGNT 100
    RAFTCPYHGW SYGINGELID VPLEPRAYPQ GLCKSHWGLN EVPCVESYKG 150
    LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR REGGTEIVGG VQKWVINCNW 200
    KSPAEQFASD QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ TARPVWETAK 250
    DALQFGQDGH GSGFFFTEKP DANVWVDGAV SSYYRETYAE AEQRLGEVRA 300
    LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK 350
    AAFENSATRA FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ 400
    EKRRDDGIPG ITNYIFSETA ARGMYQRWAD LLSSESWQEV LDKTAAYQQE 450
    VMK 453
    Length:453
    Mass (Da):51,049
    Last modified:December 6, 2005 - v1
    Checksum:i43535BF0F57643F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000036 Genomic DNA. Translation: ABB67106.1.
    RefSeqiYP_408934.1. NC_007613.1.

    Genome annotation databases

    EnsemblBacteriaiABB67106; ABB67106; SBO_2562.
    GeneIDi3781545.
    KEGGisbo:SBO_2562.
    PATRICi18684554. VBIShiBoy33460_3184.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000036 Genomic DNA. Translation: ABB67106.1 .
    RefSeqi YP_408934.1. NC_007613.1.

    3D structure databases

    ProteinModelPortali Q31XV2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300268.SBO_2562.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB67106 ; ABB67106 ; SBO_2562 .
    GeneIDi 3781545.
    KEGGi sbo:SBO_2562.
    PATRICi 18684554. VBIShiBoy33460_3184.

    Phylogenomic databases

    eggNOGi COG4638.
    HOGENOMi HOG000105925.
    KOi K05708.
    OMAi KAEWMMH.
    OrthoDBi EOG6W19DT.

    Enzyme and pathway databases

    UniPathwayi UPA00714 .
    BioCyci SBOY300268:GJFL-2559-MONOMER.

    Family and domain databases

    Gene3Di 2.102.10.10. 1 hit.
    3.90.380.10. 1 hit.
    HAMAPi MF_01648. HcaE.
    InterProi IPR020875. HcaE.
    IPR017941. Rieske_2Fe-2S.
    IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
    IPR015879. Ring_hydroxy_dOase_asu_C_dom.
    IPR001663. Rng_hydr_dOase-A.
    [Graphical view ]
    Pfami PF00355. Rieske. 1 hit.
    PF00848. Ring_hydroxyl_A. 1 hit.
    [Graphical view ]
    PRINTSi PR00090. RNGDIOXGNASE.
    SUPFAMi SSF50022. SSF50022. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    PS00570. RING_HYDROXYL_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sb227.

    Entry informationi

    Entry nameiHCAE_SHIBS
    AccessioniPrimary (citable) accession number: Q31XV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3