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Q31XV2 (HCAE_SHIBS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha

EC=1.14.12.19
Alternative name(s):
Digoxigenin subunit alpha
Gene names
Name:hcaE
Ordered Locus Names:SBO_2562
OrganismShigella boydii serotype 4 (strain Sb227) [Complete proteome] [HAMAP]
Taxonomic identifier300268 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP-Rule MF_01648

Catalytic activity

3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+. HAMAP-Rule MF_01648

(2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+. HAMAP-Rule MF_01648

Cofactor

Binds 1 iron ion By similarity. HAMAP-Rule MF_01648

Binds 1 2Fe-2S cluster per subunit By similarity. HAMAP-Rule MF_01648

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01648

Subunit structure

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD) By similarity.

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

Contains 1 Rieske domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4534533-phenylpropionate/cinnamic acid dioxygenase subunit alpha HAMAP-Rule MF_01648
PRO_0000333706

Regions

Domain44 – 14299Rieske

Sites

Metal binding851Iron-sulfur (2Fe-2S) By similarity
Metal binding871Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal binding1051Iron-sulfur (2Fe-2S) By similarity
Metal binding1081Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal binding2131Iron By similarity
Metal binding2181Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q31XV2 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 43535BF0F57643F4

FASTA45351,049
        10         20         30         40         50         60 
MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES QIPKPGDFFN 

        70         80         90        100        110        120 
TYMGEDAVVV VRQKDGSIKA FLNQCRHRAM RVSYADCGNT RAFTCPYHGW SYGINGELID 

       130        140        150        160        170        180 
VPLEPRAYPQ GLCKSHWGLN EVPCVESYKG LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR 

       190        200        210        220        230        240 
REGGTEIVGG VQKWVINCNW KSPAEQFASD QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ 

       250        260        270        280        290        300 
TARPVWETAK DALQFGQDGH GSGFFFTEKP DANVWVDGAV SSYYRETYAE AEQRLGEVRA 

       310        320        330        340        350        360 
LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK AAFENSATRA 

       370        380        390        400        410        420 
FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ EKRRDDGIPG ITNYIFSETA 

       430        440        450 
ARGMYQRWAD LLSSESWQEV LDKTAAYQQE VMK 

« Hide

References

[1]"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery."
Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. expand/collapse author list , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q.
Nucleic Acids Res. 33:6445-6458(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sb227.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000036 Genomic DNA. Translation: ABB67106.1.
RefSeqYP_408934.1. NC_007613.1.

3D structure databases

ProteinModelPortalQ31XV2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300268.SBO_2562.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB67106; ABB67106; SBO_2562.
GeneID3781545.
KEGGsbo:SBO_2562.
PATRIC18684554. VBIShiBoy33460_3184.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4638.
HOGENOMHOG000105925.
KOK05708.
OMAKAEWMMH.
OrthoDBEOG6W19DT.
ProtClustDBCLSK880425.

Enzyme and pathway databases

BioCycSBOY300268:GJFL-2559-MONOMER.
UniPathwayUPA00714.

Family and domain databases

Gene3D2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
HAMAPMF_01648. HcaE.
InterProIPR020875. HcaE.
IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSPR00090. RNGDIOXGNASE.
SUPFAMSSF50022. SSF50022. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHCAE_SHIBS
AccessionPrimary (citable) accession number: Q31XV2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 6, 2005
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways