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Q31XV2

- HCAE_SHIBS

UniProt

Q31XV2 - HCAE_SHIBS

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Protein

3-phenylpropionate/cinnamic acid dioxygenase subunit alpha

Gene
hcaE, SBO_2562
Organism
Shigella boydii serotype 4 (strain Sb227)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity.UniRule annotation

Catalytic activityi

3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+.UniRule annotation
(2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.UniRule annotation

Cofactori

Binds 1 iron ion By similarity.UniRule annotation
Binds 1 2Fe-2S cluster per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron-sulfur (2Fe-2S) By similarity
Metal bindingi87 – 871Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal bindingi105 – 1051Iron-sulfur (2Fe-2S) By similarity
Metal bindingi108 – 1081Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal bindingi213 – 2131Iron By similarity
Metal bindingi218 – 2181Iron By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 3-phenylpropionate dioxygenase activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciSBOY300268:GJFL-2559-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha (EC:1.14.12.19)
Alternative name(s):
Digoxigenin subunit alpha
Gene namesi
Name:hcaE
Ordered Locus Names:SBO_2562
OrganismiShigella boydii serotype 4 (strain Sb227)
Taxonomic identifieri300268 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000007067: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4534533-phenylpropionate/cinnamic acid dioxygenase subunit alphaUniRule annotationPRO_0000333706Add
BLAST

Interactioni

Subunit structurei

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD) By similarity.

Protein-protein interaction databases

STRINGi300268.SBO_2562.

Structurei

3D structure databases

ProteinModelPortaliQ31XV2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 14299RieskeAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.

Phylogenomic databases

eggNOGiCOG4638.
HOGENOMiHOG000105925.
KOiK05708.
OMAiKAEWMMH.
OrthoDBiEOG6W19DT.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
HAMAPiMF_01648. HcaE.
InterProiIPR020875. HcaE.
IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q31XV2-1 [UniParc]FASTAAdd to Basket

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MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES    50
QIPKPGDFFN TYMGEDAVVV VRQKDGSIKA FLNQCRHRAM RVSYADCGNT 100
RAFTCPYHGW SYGINGELID VPLEPRAYPQ GLCKSHWGLN EVPCVESYKG 150
LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR REGGTEIVGG VQKWVINCNW 200
KSPAEQFASD QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ TARPVWETAK 250
DALQFGQDGH GSGFFFTEKP DANVWVDGAV SSYYRETYAE AEQRLGEVRA 300
LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK 350
AAFENSATRA FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ 400
EKRRDDGIPG ITNYIFSETA ARGMYQRWAD LLSSESWQEV LDKTAAYQQE 450
VMK 453
Length:453
Mass (Da):51,049
Last modified:December 6, 2005 - v1
Checksum:i43535BF0F57643F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000036 Genomic DNA. Translation: ABB67106.1.
RefSeqiYP_408934.1. NC_007613.1.

Genome annotation databases

EnsemblBacteriaiABB67106; ABB67106; SBO_2562.
GeneIDi3781545.
KEGGisbo:SBO_2562.
PATRICi18684554. VBIShiBoy33460_3184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000036 Genomic DNA. Translation: ABB67106.1 .
RefSeqi YP_408934.1. NC_007613.1.

3D structure databases

ProteinModelPortali Q31XV2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300268.SBO_2562.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB67106 ; ABB67106 ; SBO_2562 .
GeneIDi 3781545.
KEGGi sbo:SBO_2562.
PATRICi 18684554. VBIShiBoy33460_3184.

Phylogenomic databases

eggNOGi COG4638.
HOGENOMi HOG000105925.
KOi K05708.
OMAi KAEWMMH.
OrthoDBi EOG6W19DT.

Enzyme and pathway databases

UniPathwayi UPA00714 .
BioCyci SBOY300268:GJFL-2559-MONOMER.

Family and domain databases

Gene3Di 2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
HAMAPi MF_01648. HcaE.
InterProi IPR020875. HcaE.
IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view ]
Pfami PF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view ]
PRINTSi PR00090. RNGDIOXGNASE.
SUPFAMi SSF50022. SSF50022. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sb227.

Entry informationi

Entry nameiHCAE_SHIBS
AccessioniPrimary (citable) accession number: Q31XV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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