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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Shigella boydii serotype 4 (strain Sb227)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei94S-adenosyl-L-methionineUniRule annotation1
Binding sitei121S-adenosyl-L-methionineUniRule annotation1
Active sitei144By similarity1
Binding sitei144S-adenosyl-L-methionineUniRule annotation1
Binding sitei148SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:SBO_3030
OrganismiShigella boydii serotype 4 (strain Sb227)
Taxonomic identifieri300268 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000007067 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291951 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ31WM2
SMRiQ31WM2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 155Interaction with RNAUniRule annotation6
Regioni217 – 220Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q31WM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQEHALEN YWPVMGVEFS
60 70 80 90 100
EDMLDFPALF GREAPVTLEI GFGMGASLVA MAKDRPEQDF LGIEVHSPGV
110 120 130 140 150
GACLASAHEE GLNNLRVMCH DAVEVLHKMI PDNSLRMVQL FFPDPWHKAR
160 170 180 190 200
HNKRRIVQVP FAELVKSKLQ LGGVFHMATD WEPYAEHMLE VMSSIDGYKN
210 220 230
LSESNDYVPR PASRPVTKFE QRGHRLGHGV WDLMFERVK
Length:239
Mass (Da):27,334
Last modified:December 6, 2005 - v1
Checksum:iA538AF9A0F08531B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000036 Genomic DNA Translation: ABB67536.1

Genome annotation databases

EnsemblBacteriaiABB67536; ABB67536; SBO_3030
KEGGisbo:SBO_3030

Similar proteinsi

Entry informationi

Entry nameiTRMB_SHIBS
AccessioniPrimary (citable) accession number: Q31WM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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