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Protein

Phosphoenolpyruvate carboxykinase (ATP)

Gene

pckA

Organism
Shigella boydii serotype 4 (strain Sb227)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65SubstrateUniRule annotation1
Binding sitei207SubstrateUniRule annotation1
Metal bindingi213ManganeseUniRule annotation1
Binding sitei213ATPUniRule annotation1
Binding sitei213SubstrateUniRule annotation1
Metal bindingi232Manganese; via tele nitrogenUniRule annotation1
Binding sitei232ATPUniRule annotation1
Metal bindingi269ManganeseUniRule annotation1
Binding sitei297ATPUniRule annotation1
Binding sitei333ATPUniRule annotation1
Binding sitei333SubstrateUniRule annotation1
Binding sitei455ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi248 – 256ATPUniRule annotation9
Nucleotide bindingi449 – 450ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase (ATP)UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:SBO_3390
OrganismiShigella boydii serotype 4 (strain Sb227)
Taxonomic identifieri300268 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000007067 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002369421 – 540Phosphoenolpyruvate carboxykinase (ATP)Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87N6-acetyllysineUniRule annotation1
Modified residuei523N6-acetyllysineUniRule annotation1

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ31VN1
SMRiQ31VN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000271471
KOiK01610
OMAiMRYAGEM

Family and domain databases

CDDicd00484 PEPCK_ATP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00453 PEPCK_ATP, 1 hit
InterProiView protein in InterPro
IPR001272 PEP_carboxykinase_ATP
IPR013035 PEP_carboxykinase_C
IPR008210 PEP_carboxykinase_N
IPR015994 PEPCK_ATP_CS
PANTHERiPTHR30031 PTHR30031, 1 hit
PfamiView protein in Pfam
PF01293 PEPCK_ATP, 1 hit
PIRSFiPIRSF006294 PEP_crbxkin, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
TIGRFAMsiTIGR00224 pckA, 1 hit
PROSITEiView protein in PROSITE
PS00532 PEPCK_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Q31VN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVNNGLTPQ ELEAYGISDV HDIVYNPSYD LLYQEELDPS LTGYERGVLT
60 70 80 90 100
NLGAVAVDTG IFTGRSPKDK YIVRDDTTRD TFWWADKGKG KNDNKPLSPE
110 120 130 140 150
TWQHLKGLVT RQLSGKRLFV VDAFCGANPD TRLSVRFITE VAWQAHFVKN
160 170 180 190 200
MFIRPSDEEL AGFKPDFIVM NGAKCTNPQW KEQGLNSENF VAFNLTERMQ
210 220 230 240 250
LIGGTWYGGE MKKGMFSMMN YLLPLKGIAS MHCSANVGEK GDVAVFFGLS
260 270 280 290 300
GTGKTTLSTD PKRRLIGDDE HGWDDDGVFN FEGGCYAKTI KLSKEAEPEI
310 320 330 340 350
YNAIRRDALL ENVTVREDGT IDFDDGSKTE NTRVSYPIYH IDNIVKPVSK
360 370 380 390 400
AGHATKVIFL TADAFGVLPP VSRLTADQTQ YHFLSGFTAK LAGTERGITE
410 420 430 440 450
PTPTFSACFG AAFLSLHPTQ YAEVLVKRMQ AAGAQAYLVN TGWNGTGKRI
460 470 480 490 500
SIKDTRAIID AILNGSLDNA ETFTLPMFNL AIPTELPGVD TKILDPRNTY
510 520 530 540
ASPEQWQEKA ETLAKLFIDN FDKYTDTPAG AALVAAGPKL
Length:540
Mass (Da):59,643
Last modified:December 6, 2005 - v1
Checksum:iA744D90899F6A8A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000036 Genomic DNA Translation: ABB67877.1

Genome annotation databases

EnsemblBacteriaiABB67877; ABB67877; SBO_3390
KEGGisbo:SBO_3390

Similar proteinsi

Entry informationi

Entry nameiPCKA_SHIBS
AccessioniPrimary (citable) accession number: Q31VN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health