Reviewed, UniProtKB/Swiss-Prot Q31NV7 (UCRI_SYNE7)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
      Short name=ISP
      Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	Synpcc7942_1232

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity. HAMAP MF_01335
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin. HAMAP MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. HAMAP MF_01335
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity. HAMAP MF_01335
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity. HAMAP MF_01335
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Cytochrome b6-f complex iron-sulfur subunit HAMAP MF_01335		PRO_0000298467
Regions
	Transmembrane	21 – 43	23	Potential
	Domain	61 – 162	102	Rieske
Sites
	Metal binding	108	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	110	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	126	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	129	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	113 ↔ 128		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q31NV7-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: EC139A4D25CAA047	FASTA	179	18,834
	10 20 30 40 50 60 MTQVSGASDV PSMGRRQFMN LLTFGSVTGV ALGALYPVVN YFIPPSSGGS GGGVAAKDAL 70 80 90 100 110 120 GNDVVLSKFL ADHNVGDRTL VQGLKGDPTY LVVESSEAIG DYGINAVCTH LGCVVPWNAS 130 140 150 160 170 ENKFKCPCHG SQYDATGKVV RGPAPLSLAL AHVSVTDDKV FLSPWTETDF RTGDNPWWA

References

[1]

"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000100 Genomic DNA. Translation: ABB57262.1.
RefSeq	YP_400249.1.
3D structure databases
HSSP	HSSP built from PDB template 2D2C based on UniProtKB P83794.
SMR	Q31NV7. Positions 9-178.
ModBase	Search...
Protein-protein interaction databases
STRING	Q31NV7.
Genome annotation databases
GeneID	3773520.
GenomeReviews	Gene locus Synpcc7942_1232 in contig CP000100_GR.
KEGG	syf:Synpcc7942_1232.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0723.
HOGENOM	HBG668715.
Enzyme and pathway databases
BioCyc	MetaCyc:SYNPCC7942_1232-MONOMER. SELO1140:SYNPCC7942_1232-MONOMER.
Family and domain databases
HAMAP	MF_01335. Cytb6_f_Rieske. [Tree]
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_SYNE7

Accession

Primary (citable) accession number: Q31NV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	December 6, 2005
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)