Reviewed, UniProtKB/Swiss-Prot Q31LY2 (ACSF_SYNE7)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	Synpcc7942_1907

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_1000070552

Sequences

	Sequence		Length	Mass (Da)
	Q31LY2-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 6C047F45E9DAB3A2	FASTA	358	42,120
	10 20 30 40 50 60 MVDSLQKPEL EELRPGIKAP AKETILTPRF YTTDFEAMAK MDLSPNEDEL RAILEEFRAD 70 80 90 100 110 120 YNRKHFVRTP EFDGSAADMD PETKKVFVEF LEQSCTSEFS GFLLYKELSR RIKNRNPLLA 130 140 150 160 170 180 ECFELMARDE ARHAGFLNKS MSDFDLQLDL GFLTANKKYT YFKPAYIFYA TYLSEKIGYW 190 200 210 220 230 240 RYITIFRHLE QHPEYRIYPI FKFFENWCQD ENRHGDFFDA LMKAQPSTVR GFVAKLWCRF 250 260 270 280 290 300 FLLAVFATMY IRDVQRKEFY EALGLDAREY DRHVIAKTNE TSARVFPVVL DVDHPKFYDR 310 320 330 340 350 LETCIANNNQ LAAIEATSAP KPVKVLRKLP YYLSNGLQLL KLYLVKPLES DVYQPAVR

References

[1]

"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000100 Genomic DNA. Translation: ABB57937.1.
RefSeq	YP_400924.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3775270.
GenomeReviews	Gene locus Synpcc7942_1907 in contig CP000100_GR.
KEGG	syf:Synpcc7942_1907.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q31LY2.
Enzyme and pathway databases
BioCyc	SELO1140:SYNPCC7942_1907-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_SYNE7

Accession

Primary (citable) accession number: Q31LY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 6, 2005
Last modified:	November 25, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)