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Q31KE9

- PNP_SYNE7

UniProt

Q31KE9 - PNP_SYNE7

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi495 – 4951MagnesiumUniRule annotation
Metal bindingi501 – 5011MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_2440-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Synpcc7942_2440
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002717: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Polyribonucleotide nucleotidyltransferasePRO_0000329901Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi1140.Synpcc7942_2440.

Structurei

3D structure databases

ProteinModelPortaliQ31KE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini562 – 62160KHUniRule annotationAdd
BLAST
Domaini631 – 69969S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q31KE9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQLSKSLSF DGRDIRLELG LFAPQAGGSV LISSGDTAVL VAATRSTARE
60 70 80 90 100
GIDFLPLTVD YEERMYAAGR IPGGFLRREG RPPERATLTS RLIDRPLRPL
110 120 130 140 150
FPSWLRDDLQ VVATTLSMDE TVPPDVLAVT GASIATLVAK IPFYGPMAAV
160 170 180 190 200
RVGLVGDDFI INPTYREIEK GDLDLVVAGT PAGVIMVEAG ANQLPEADVI
210 220 230 240 250
EAIDFGYEAV QELIKAQQSL LAELGIEQIL PEAPNADNTL ENFVRDRASS
260 270 280 290 300
GVQQVLQHFS FTKSERDAAL DEVKASVVEA IAALPEEDPV RSVTAAEPKA
310 320 330 340 350
LGNTFKALTK TLMRQQILRD GVRVDGRKLD QVRPISSQVG LLPPRVHGSG
360 370 380 390 400
LFQRGLTQVL SIATLGTPGD AQELDDLHPD TQKRYLHHYN MPPYSVGETR
410 420 430 440 450
PMRSPGRREI GHGALAERAL LPVLPSKEEF PYVIRVVSEV LSSNGSTSMG
460 470 480 490 500
SVCGSTLALM DAGVPIKKPV SGAAMGLIRE GDEYRVLTDI QGIEDFLGDM
510 520 530 540 550
DFKVAGTDQG ITALQMDMKI HGLPLEIIAD AINQAKPARL HILNKMLEAI
560 570 580 590 600
ATPRADLSTY APRLFRIQIN PEQIGLVIGP GGKTIRSITE QTGAKIDIED
610 620 630 640 650
TGAVTISAVD ADSALRAKSI IEGMTRTITA GDVYIGKVTR IIPIGAFVEF
660 670 680 690 700
LPGKEGMIHI SQIADYRVAR VEDELTVGDE VVVKVREIDQ KGRVNLTRKG
710
IDPEEVSAAR AAVEAS
Length:716
Mass (Da):77,193
Last modified:December 6, 2005 - v1
Checksum:i4B7A1FA3C37C05E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000100 Genomic DNA. Translation: ABB58470.1.
RefSeqiYP_401457.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB58470; ABB58470; Synpcc7942_2440.
GeneIDi3774459.
KEGGisyf:Synpcc7942_2440.
PATRICi23790445. VBISynElo51371_2735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000100 Genomic DNA. Translation: ABB58470.1 .
RefSeqi YP_401457.1. NC_007604.1.

3D structure databases

ProteinModelPortali Q31KE9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 1140.Synpcc7942_2440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB58470 ; ABB58470 ; Synpcc7942_2440 .
GeneIDi 3774459.
KEGGi syf:Synpcc7942_2440.
PATRICi 23790445. VBISynElo51371_2735.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci SYNEL:SYNPCC7942_2440-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7942.

Entry informationi

Entry nameiPNP_SYNE7
AccessioniPrimary (citable) accession number: Q31KE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 6, 2005
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3