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Q31JF8 (TRMB_THICR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Tcr_0119
OrganismThiomicrospira crunogena (strain XCL-2) [Complete proteome] [HAMAP]
Taxonomic identifier317025 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeThiomicrospira

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229207

Regions

Region219 – 2224Substrate binding By similarity

Sites

Active site1461 By similarity
Binding site711S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1231S-adenosyl-L-methionine By similarity
Binding site1461S-adenosyl-L-methionine By similarity
Binding site1501Substrate By similarity
Binding site1821Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q31JF8 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 2B61430A124B7CAB

FASTA24027,349
        10         20         30         40         50         60 
MTENTESPQS VATDIPEHKR RIKSFVLRQG RLSQAQQNAI DTMWPQYGLT LEDKLLDFNT 

        70         80         90        100        110        120 
LFGREAPTII EIGFGMGNSL AAMAEAHPEN NYIGIEVHRP GVGALLKLVA EKGLTNVRVF 

       130        140        150        160        170        180 
NEDAIEVLNR QIPKNSLSAV YLFFPDPWHK TKHKKRRILQ AEFAEKIAQY LAPGGQFHMA 

       190        200        210        220        230        240 
TDWEDYALHM MSVMSAAKGY RNISGEGQYT PRPDYRPLTK FEQRGHRLGH GVWDLVFERI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000109 Genomic DNA. Translation: ABB40715.1.
RefSeqYP_390389.1. NC_007520.2.

3D structure databases

ProteinModelPortalQ31JF8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING317025.Tcr_0119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB40715; ABB40715; Tcr_0119.
GeneID3762527.
KEGGtcx:Tcr_0119.
PATRIC23972106. VBIThiCru83387_0125.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycTCRU317025:GHE8-119-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_THICR
AccessionPrimary (citable) accession number: Q31JF8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 6, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways