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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Hydrogenovibrio crunogenus (strain XCL-2) (Thiomicrospira crunogena)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Binding sitei123S-adenosyl-L-methionineUniRule annotation1
Active sitei146By similarity1
Binding sitei146S-adenosyl-L-methionineUniRule annotation1
Binding sitei150SubstrateUniRule annotation1
Binding sitei182SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Tcr_0119
OrganismiHydrogenovibrio crunogenus (strain XCL-2) (Thiomicrospira crunogena)
Taxonomic identifieri317025 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeHydrogenovibrio
Proteomesi
  • UP000002713 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002292071 – 240tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST240

Interactioni

Protein-protein interaction databases

STRINGi317025.Tcr_0119.

Structurei

3D structure databases

ProteinModelPortaliQ31JF8.
SMRiQ31JF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 222Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiPDPWHKS.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q31JF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENTESPQS VATDIPEHKR RIKSFVLRQG RLSQAQQNAI DTMWPQYGLT
60 70 80 90 100
LEDKLLDFNT LFGREAPTII EIGFGMGNSL AAMAEAHPEN NYIGIEVHRP
110 120 130 140 150
GVGALLKLVA EKGLTNVRVF NEDAIEVLNR QIPKNSLSAV YLFFPDPWHK
160 170 180 190 200
TKHKKRRILQ AEFAEKIAQY LAPGGQFHMA TDWEDYALHM MSVMSAAKGY
210 220 230 240
RNISGEGQYT PRPDYRPLTK FEQRGHRLGH GVWDLVFERI
Length:240
Mass (Da):27,349
Last modified:December 6, 2005 - v1
Checksum:i2B61430A124B7CAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000109 Genomic DNA. Translation: ABB40715.1.
RefSeqiWP_011369541.1. NC_007520.2.

Genome annotation databases

EnsemblBacteriaiABB40715; ABB40715; Tcr_0119.
KEGGitcx:Tcr_0119.

Similar proteinsi

Entry informationi

Entry nameiTRMB_HYDCU
AccessioniPrimary (citable) accession number: Q31JF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 6, 2005
Last modified: November 22, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families