Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q31IE8 (SYL_THICR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Tcr_0479
OrganismThiomicrospira crunogena (strain XCL-2) [Complete proteome] [HAMAP]
Taxonomic identifier317025 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeThiomicrospira

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 877877Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334835

Regions

Motif50 – 6011"HIGH" region HAMAP-Rule MF_00049
Motif634 – 6385"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q31IE8 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 8721947CB36B912F

FASTA87798,347
        10         20         30         40         50         60 
MTTESMPEST NDYQPQSIEA AIQHVWDEQQ VFVAKEDDSK EKFYCLSMFP YPSGKLHMGH 

        70         80         90        100        110        120 
VRNYTIGDVV SRFQRMQGKN VLQPMGWDAF GLPAENAAMD NQVAPAKWTY QNIDYMRNQL 

       130        140        150        160        170        180 
KRLGLGYDWT REVATCHPEY YRWEQWLFTR LMEKGLVYRK LSVVNWDPVD QTVLANEQVI 

       190        200        210        220        230        240 
DGKGWRSGVP VERKEIAQWF LRITDYAEEL LTDLDQLEGW PEQVKTMQKN WIGKSTGLEI 

       250        260        270        280        290        300 
EFPIASGLDE SNGSLKVYTT RPDTLMGVTY VAVAADHPWA RKASVNNEPL ERFIEECSHI 

       310        320        330        340        350        360 
STAEADMETM EKKGVDTGIR VKHPITGDEV PVWAANFVLM GYGTGAVMSV PAHDQRDYEF 

       370        380        390        400        410        420 
AKAYDLPIKA VIAPKAGEEA DVSEAAFTEK GVLVNSGQFD GLKSKQALHE MAKVLGELGL 

       430        440        450        460        470        480 
GEKQTNYRLR DWGISRQRYW GCPIPVIYCP ACGALPVPEK DLPVRLPEDV VPDGSGSPLA 

       490        500        510        520        530        540 
KLDSFKKCEC PQCGGPANRE TDTFDTFFES SWYHARYTSR HEDNAMLDKA AADHWLPVDQ 

       550        560        570        580        590        600 
YIGGIEHAIL HLLYARFFHK LMRDEGLVSS DEPFKNLLTQ GMVLAGSWFT QDEKGKQTWY 

       610        620        630        640        650        660 
SPLDVDPVTD DKGAIVKGTL KSDGTEVQYG GIIKMSKSKN NGIDPQTLID QYGADTLRLY 

       670        680        690        700        710        720 
IMFASPPEQT LEWSDSAVEG AHRFLNRVWR QVQTHVSTGV VAACTSNDDL TKEQKALRLK 

       730        740        750        760        770        780 
LHTTLQKVTD DMGRRMHFNT AIAATMELLN DISRFKDESD AGRSVMQEAL EMLVLMLSPM 

       790        800        810        820        830        840 
TPHASQALWE ALGHDGLVLN VTWPTVDDAA LVKDEIEIMV QVNGKLRGKI EVAAEADKDT 

       850        860        870 
ILAAAKANEQ AAKFIDGKDI VKEIVVPGRL VNIVVKG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000109 Genomic DNA. Translation: ABB41075.1.
RefSeqYP_390749.1. NC_007520.2.

3D structure databases

ProteinModelPortalQ31IE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING317025.Tcr_0479.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB41075; ABB41075; Tcr_0479.
GeneID3761580.
KEGGtcx:Tcr_0479.
PATRIC23972850. VBIThiCru83387_0491.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycTCRU317025:GHE8-485-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THICR
AccessionPrimary (citable) accession number: Q31IE8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries