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Q31H85 (RUVC_THICR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Tcr_0892
OrganismThiomicrospira crunogena (strain XCL-2) [Complete proteome] [HAMAP]
Taxonomic identifier317025 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeThiomicrospira

Protein attributes

Sequence length172 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 172172Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000071036

Sites

Metal binding111Magnesium By similarity
Metal binding701Magnesium By similarity
Metal binding1421Magnesium By similarity
Metal binding1451Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q31H85 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: CE549F44D875B897

FASTA17218,886
        10         20         30         40         50         60 
MAQLKRILGI DPGSRKTGFG IIESGRFHPN YVSSGVIRVE KLTGAQRLKT IFESVCQIID 

        70         80         90        100        110        120 
QYQPHVMAIE KVFVYKNPSS AIKLGQARGV ILCAAAIKEI PIMEYTPTQI KSTIVGQGHA 

       130        140        150        160        170 
TKDQVQFMVQ NLLKLTESPQ EDAADALAGA LCHDRYLTLG IDPEKISKGT KF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000109 Genomic DNA. Translation: ABB41488.1.
RefSeqYP_391162.1. NC_007520.2.

3D structure databases

ProteinModelPortalQ31H85.
SMRQ31H85. Positions 6-157.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING317025.Tcr_0892.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB41488; ABB41488; Tcr_0892.
GeneID3761363.
KEGGtcx:Tcr_0892.
PATRIC23973742. VBIThiCru83387_0928.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMARQVQEMV.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycTCRU317025:GHE8-911-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVC_THICR
AccessionPrimary (citable) accession number: Q31H85
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families