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Q31GG8 (CYSG_THICR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Siroheme synthase

Including the following 3 domains:

  1. Uroporphyrinogen-III C-methyltransferase
    Short name=Urogen III methylase
    EC=2.1.1.107
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name=UROM
  2. Precorrin-2 dehydrogenase
    EC=1.3.1.76
  3. Sirohydrochlorin ferrochelatase
    EC=4.99.1.4
Gene names
Name:cysG
Ordered Locus Names:Tcr_1160
OrganismThiomicrospira crunogena (strain XCL-2) [Complete proteome] [HAMAP]
Taxonomic identifier317025 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeThiomicrospira

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity. HAMAP-Rule MF_01646

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP-Rule MF_01646

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP-Rule MF_01646

Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP-Rule MF_01646

Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP-Rule MF_01646

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646

Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.

Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.

In the C-terminal section; belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Siroheme synthase HAMAP-Rule MF_01646
PRO_0000330566

Regions

Nucleotide binding22 – 232NAD By similarity
Nucleotide binding43 – 442NAD By similarity
Region1 – 206206precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Region223 – 473251Uroporphyrinogen-III C-methyltransferase By similarity
Region308 – 3103S-adenosyl-L-methionine binding By similarity
Region338 – 3392S-adenosyl-L-methionine binding By similarity

Sites

Active site2551Proton acceptor By similarity
Active site2771Proton donor By similarity
Binding site2321S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site3131S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site3901S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site4191S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q31GG8 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: A05B84E840C6E353

FASTA47351,899
        10         20         30         40         50         60 
MDYLPIFMKI EQQHCLIVGG GTVAARKADL FIKSGAIVTV VAPKLGNEMT FHLAQGKIIW 

        70         80         90        100        110        120 
HMNTFSTALV SELPRPSLVI SATDDQNVNL AVYKTYHAQD IPVNVADQTE YCDFILPAIV 

       130        140        150        160        170        180 
DRSPMTIAIS TGGRSPVLAR VMKARLETMI PHGFSVLTDL VGRYRQTVKN VISDIDGRKT 

       190        200        210        220        230        240 
FWETLLSGLF IDKAVHGNTG EAEALLEAEL ETIKNNGQSL PQGEVYIIGA GPGDPDLMTF 

       250        260        270        280        290        300 
KGLRLLQQAD VILYDRLVAP EILEMGRREA ERIYVGKKEK WHKMDQKDIN QMLVDLARQG 

       310        320        330        340        350        360 
KRVARLKGGD PYIFGRGAEE VELLVQHDVP YQVVPGITAA AGCSVYADFP LTHRDYAQSV 

       370        380        390        400        410        420 
ALITGHQQAG AQGIDYARLA QSGDTMVFYM GIKNAPKIQA GLIAHGLCPD TPAAIIENGT 

       430        440        450        460        470 
RLNQKVTVTS LAKLSETIQK KSIKPPALLV IGEVIKVRER LQKKSLLDDR VPA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000109 Genomic DNA. Translation: ABB41755.1.
RefSeqYP_391429.1. NC_007520.2.

3D structure databases

ProteinModelPortalQ31GG8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING317025.Tcr_1160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB41755; ABB41755; Tcr_1160.
GeneID3760647.
KEGGtcx:Tcr_1160.
PATRIC23974316. VBIThiCru83387_1210.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0007.
HOGENOMHOG000290518.
KOK02302.
OMAQASFIMP.
OrthoDBEOG6DRPFR.

Enzyme and pathway databases

BioCycTCRU317025:GHE8-1185-MONOMER.
UniPathwayUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Family and domain databases

Gene3D1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_01646. Siroheme_synth.
InterProIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMSSF53790. SSF53790. 1 hit.
TIGRFAMsTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEPS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSG_THICR
AccessionPrimary (citable) accession number: Q31GG8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways