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Q31F42

- LIPA_THICR

UniProt

Q31F42 - LIPA_THICR

Protein

Lipoyl synthase

Gene

lipA

Organism
Thiomicrospira crunogena (strain XCL-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciTCRU317025:GHE8-1682-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Tcr_1639
    OrganismiThiomicrospira crunogena (strain XCL-2)
    Taxonomic identifieri317025 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeThiomicrospira
    ProteomesiUP000002713: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316Lipoyl synthasePRO_0000325319Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi317025.Tcr_1639.

    Structurei

    3D structure databases

    ProteinModelPortaliQ31F42.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q31F42-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKARNESMSK GEYKTKSLKN RPDPTQPKLK KPSWIKAKLP SAKHIGRVKE    50
    LKQVLREQGL NSVCEEASCP NLGECFGHGT ATFMIMGHIC TRKCPFCDVT 100
    HGRPNPLNQD EPRHLAKTIH AMNLNYVVIT SVDRDDLRDG GATHFKNCTQ 150
    AIRDKMPDIQ IETLVPDFRG RLTVALDILA QQAPDVLNHN LETVPRLYEE 200
    ARPGADYQAS LDLLKRFKQM VPETKTKSGL MVGLGETFDE ILQVMRDLRA 250
    HDVEMLTVGQ YLQPSDFHLA VQRYWTPEEF KQLEQAGMEM GFTHVASGPM 300
    VRSSYHADLQ AQGQFS 316
    Length:316
    Mass (Da):35,700
    Last modified:March 18, 2008 - v2
    Checksum:i2025EABFFB8E7A8E
    GO

    Sequence cautioni

    The sequence ABB42231.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000109 Genomic DNA. Translation: ABB42231.1. Different initiation.
    RefSeqiYP_391905.1. NC_007520.2.

    Genome annotation databases

    EnsemblBacteriaiABB42231; ABB42231; Tcr_1639.
    GeneIDi3761936.
    KEGGitcx:Tcr_1639.
    PATRICi23975352. VBIThiCru83387_1712.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000109 Genomic DNA. Translation: ABB42231.1 . Different initiation.
    RefSeqi YP_391905.1. NC_007520.2.

    3D structure databases

    ProteinModelPortali Q31F42.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 317025.Tcr_1639.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB42231 ; ABB42231 ; Tcr_1639 .
    GeneIDi 3761936.
    KEGGi tcx:Tcr_1639.
    PATRICi 23975352. VBIThiCru83387_1712.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci TCRU317025:GHE8-1682-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: XCL-2.

    Entry informationi

    Entry nameiLIPA_THICR
    AccessioniPrimary (citable) accession number: Q31F42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3