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Q31EF5 (LEU12_THICR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-isopropylmalate synthase 2
EC=2.3.3.13
Alternative name(s):
Alpha-IPM synthase 2
Alpha-isopropylmalate synthase 2
Gene names
Name:leuA2
Ordered Locus Names:Tcr_1878
OrganismThiomicrospira crunogena (strain XCL-2) [Complete proteome] [HAMAP]
Taxonomic identifier317025 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Piscirickettsiaceae › Thiomicrospira ›

Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_00572

Catalytic activity

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_00572

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_00572

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_function2-isopropylmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5395392-isopropylmalate synthase 2 HAMAP-Rule MF_00572
PRO_0000406880

Sequences

Sequence LengthMass (Da)Tools
Q31EF5 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 897FA028839E99FE

FASTA53960,659
        10         20         30         40         50         60 
MTPEKYRPTI TPDLPNRQWP NQRLTQAPIW ASVDLRDGNQ ALANPMTVEQ KLKLWDQLVA 

        70         80         90        100        110        120 
IGFKTIEIGF PAASQLEFDF ARRLIEENRI PDDVTVQVLV QAREHLIKRT YEALQGVKQA 

       130        140        150        160        170        180 
VVHVYNTTST VQREKVFCKS KADIKAMAIQ GAKWVQSYAK EHPESDWVFQ YSPESFSQTE 

       190        200        210        220        230        240 
TDYAVEVCQA VMDVWQPTPD NRCILNLPAT VESTSPNRFA DQVEYFITHL KNRESAIISI 

       250        260        270        280        290        300 
HTHNDRGCAV AAAELSLLAG ADRVEGTLLG NGERTGNMDI VTLAMNLYSE GIDPELNLSD 

       310        320        330        340        350        360 
PDSWVPVIEE VTKIDTHVRH PWVGEAVYTA YSGSHQDAIR KCLMRQKDDE PWNVAYLPID 

       370        380        390        400        410        420 
PKDLNRSYEA IIRVNSQSGK AGAAFVLTQE YDLNLPKWVQ QDFAPVAQTI AEQAGGIVSH 

       430        440        450        460        470        480 
QMLYDAFIQH YRLDKSLAEL NNYQLSRKEG KEHLSVEVNG ETWQGQGNGT LSALCDAWQR 

       490        500        510        520        530 
RTGQQVDVLD YSEHALHEGK DSKAIAYVYV QQKNTKTIGI AMAEDTVSAM IQALLSTIR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000109 Genomic DNA. Translation: ABB42468.1.
RefSeqYP_392142.1. NC_007520.2.

3D structure databases

ProteinModelPortalQ31EF5.
ModBaseSearch...

Protein-protein interaction databases

STRING317025.Tcr_1878.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB42468; ABB42468; Tcr_1878.
GeneID3762414.
KEGGtcx:Tcr_1878.
PATRIC23975860. VBIThiCru83387_1958.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000110941.
KOK01649.
OMAIHTHNDR.
ProtClustDBPRK03739.

Enzyme and pathway databases

UniPathwayUPA00048; UER00070.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00572. LeuA_type2.
InterProIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005668. IPM_Synthase.
IPR000891. PYR_CT.
[Graphical view]
PfamPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMSSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
PROSITEPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU12_THICR
AccessionPrimary (citable) accession number: Q31EF5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: December 6, 2005
Last modified: May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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