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Q31E55

- BIOB_THICR

UniProt

Q31E55 - BIOB_THICR

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Protein
Biotin synthase
Gene
bioB, Tcr_1978
Organism
Thiomicrospira crunogena (strain XCL-2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi264 – 2641Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTCRU317025:GHE8-2034-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Tcr_1978
OrganismiThiomicrospira crunogena (strain XCL-2)
Taxonomic identifieri317025 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesPiscirickettsiaceaeThiomicrospira
ProteomesiUP000002713: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Biotin synthaseUniRule annotation
PRO_0000381689Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi317025.Tcr_1978.

Structurei

3D structure databases

ProteinModelPortaliQ31E55.
SMRiQ31E55. Positions 7-318.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q31E55-1 [UniParc]FASTAAdd to Basket

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MALGDVRNDW TLEEIKDIMN QPFNDLMFQA QTVHRQHFNP NEIQTSTLVN    50
IKSGGCAEDC AYCSQSARNQ TEVEKEQMME VQEVIEQAMA AKEKGATRLC 100
MGAAWRNPTK KDFPRVLEMV RVVKDLGLET CLTLGMLNDD QVKQLKEAGL 150
DYYNHNLDTS EAFYPKIITT RSYQDRLDTI DKVQEAGINV CSGGIIGMGE 200
QHKDRAELIR TFGTMRHHPN SVPINLLVPV EGTPLAHMKD KTDSFEFIRV 250
IATARIVMPQ TYVRLSAGRM ELTDEAQALC FLAGANSIFY GDKLLTTDNP 300
EAGHDEQLFA KLGIQMQQNA KVEAAAKKLA EQTTELTA 338
Length:338
Mass (Da):37,862
Last modified:December 6, 2005 - v1
Checksum:iE7968F2E03B7E966
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000109 Genomic DNA. Translation: ABB42568.1.
RefSeqiWP_011371395.1. NC_007520.2.
YP_392242.1. NC_007520.2.

Genome annotation databases

EnsemblBacteriaiABB42568; ABB42568; Tcr_1978.
GeneIDi3761178.
KEGGitcx:Tcr_1978.
PATRICi23976080. VBIThiCru83387_2062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000109 Genomic DNA. Translation: ABB42568.1 .
RefSeqi WP_011371395.1. NC_007520.2.
YP_392242.1. NC_007520.2.

3D structure databases

ProteinModelPortali Q31E55.
SMRi Q31E55. Positions 7-318.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 317025.Tcr_1978.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB42568 ; ABB42568 ; Tcr_1978 .
GeneIDi 3761178.
KEGGi tcx:Tcr_1978.
PATRICi 23976080. VBIThiCru83387_2062.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci TCRU317025:GHE8-2034-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: XCL-2.

Entry informationi

Entry nameiBIOB_THICR
AccessioniPrimary (citable) accession number: Q31E55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 6, 2005
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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