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Q31CU4

- SYI_PROM9

UniProt

Q31CU4 - SYI_PROM9

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9312)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
Binding sitei626 – 6261ATPUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation
Metal bindingi956 – 9561ZincUniRule annotation
Metal bindingi959 – 9591ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR74546:GHRG-246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PMT9312_0240
OrganismiProchlorococcus marinus (strain MIT 9312)
Taxonomic identifieri74546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002715: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligasePRO_1000022100Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi74546.PMT9312_0240.

Structurei

3D structure databases

ProteinModelPortaliQ31CU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi623 – 6275"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q31CU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSQNNKEYK SEFSYKETLN LLKTDFSMRA NSVLREPEIQ NFWAKNNIDF
60 70 80 90 100
ELGSNNSGKI FTLHDGPPYA NGALHMGHAL NKVLKDIINK YKTLRGFRVH
110 120 130 140 150
YVPGWDCHGL PIELKVLQNL KSSERKNLDT LNLRKKATDY AYVQINNQME
160 170 180 190 200
GFKRWGIWGN WDNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPDEHYSK SIYVSLKITK LSDEILLKFY QENPNFKKDF
260 270 280 290 300
FLSNSFITIW TTTPWTIPAN EAVAVNPKIN YVFAIDEEKR IYLLAKELSS
310 320 330 340 350
EISNKFNKDL TTLLEVKGVT LEDIEYQHPT KNKNCRIVIG GDYITIESGT
360 370 380 390 400
GIVHTAPGHG IDDFNVGRKY DLPTTCVVDE KGNLNEYSGQ FQGSNVLKDA
410 420 430 440 450
NDLIIDYLEE KDLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVNGFR
460 470 480 490 500
SSALKAIEDV EWIPATGKKR IYSMVVGRGD WCISRQRSWG LPIPVFYKKN
510 520 530 540 550
GNEILLNSEI INHIQKLFSE HGADIWWDWD VKHLLPDNYV KESDLWKKGT
560 570 580 590 600
DTMDVWFDSG SSWAAVCEQR SELKYPADLY LEGSDQHRGW FQSSLLTSVA
610 620 630 640 650
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKIEPAY
660 670 680 690 700
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD
710 720 730 740 750
PNIDSFEIDQ LPLLDQWMLG RLVEVTDQIS NAYENYEFSK FFQILQSFCV
760 770 780 790 800
VDLSNFYLDI AKDRLYVSSK SQFRRRSCQF VMSKVVENLA VLISPVLCHM
810 820 830 840 850
AEDIWQNVPY STKEKSVFQR GWPNFSQSWK NEILNEHIAN LRNLRVEINK
860 870 880 890 900
AIEGCRNKQI IGAALETEVN YLPKDKVVKD SLTWLKKFGN EEVDLFRDWL
910 920 930 940 950
IVSNFQVVSE LAKNSLIIDN NEIGKIQILK AHGQKCDRCW HYQEEIFSGI
960
QNTKLCKRCS HIINLEFT
Length:968
Mass (Da):111,880
Last modified:December 6, 2005 - v1
Checksum:i7C924A4490B4BEDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000111 Genomic DNA. Translation: ABB49301.1.
RefSeqiWP_011375805.1. NC_007577.1.
YP_396737.1. NC_007577.1.

Genome annotation databases

EnsemblBacteriaiABB49301; ABB49301; PMT9312_0240.
GeneIDi3765026.
KEGGipmi:PMT9312_0240.
PATRICi23004102. VBIProMar70153_0245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000111 Genomic DNA. Translation: ABB49301.1 .
RefSeqi WP_011375805.1. NC_007577.1.
YP_396737.1. NC_007577.1.

3D structure databases

ProteinModelPortali Q31CU4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 74546.PMT9312_0240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB49301 ; ABB49301 ; PMT9312_0240 .
GeneIDi 3765026.
KEGGi pmi:PMT9312_0240.
PATRICi 23004102. VBIProMar70153_0245.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR74546:GHRG-246-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Prochlorococcus marinus str. MIT 9312."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9312.

Entry informationi

Entry nameiSYI_PROM9
AccessioniPrimary (citable) accession number: Q31CU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 6, 2005
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3