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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Prochlorococcus marinus (strain MIT 9312)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35S-adenosyl-L-methionineUniRule annotation1
Binding sitei60S-adenosyl-L-methionineUniRule annotation1
Binding sitei87S-adenosyl-L-methionineUniRule annotation1
Active sitei113By similarity1
Binding sitei113S-adenosyl-L-methionineUniRule annotation1
Binding sitei117SubstrateUniRule annotation1
Binding sitei149SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PMT9312_0242
OrganismiProchlorococcus marinus (strain MIT 9312)
Taxonomic identifieri74546 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000002715 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291821 – 209tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST209

Interactioni

Protein-protein interaction databases

STRINGi74546.PMT9312_0242.

Structurei

3D structure databases

ProteinModelPortaliQ31CU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107YD0. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000237936.
KOiK03439.
OMAiFERIYIK.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q31CU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQHVNPLSR NFNEIERIPA LIEMFGDSKS NLHLDIGCAA GEFLFDLALI
60 70 80 90 100
NTNWNYIGIE IRERLVKTAK LKVRERDIKN LYFIFGNANN ILHDVQSKFI
110 120 130 140 150
FKNVKSISFN FPDPWFKKRH YKRRVIQPEF LNLLSNSLQK GSLIFIKTDV
160 170 180 190 200
KDLFDYMDCI ISNNFNFKKI DKNDFNFSES FNPSQVKTNR EKYVIVNQLD

IFERIYIKI
Length:209
Mass (Da):24,841
Last modified:December 6, 2005 - v1
Checksum:iCDD89C53629C07E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000111 Genomic DNA. Translation: ABB49303.1.
RefSeqiWP_011375807.1. NC_007577.1.

Genome annotation databases

EnsemblBacteriaiABB49303; ABB49303; PMT9312_0242.
KEGGipmi:PMT9312_0242.
PATRICi23004106. VBIProMar70153_0247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000111 Genomic DNA. Translation: ABB49303.1.
RefSeqiWP_011375807.1. NC_007577.1.

3D structure databases

ProteinModelPortaliQ31CU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi74546.PMT9312_0242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB49303; ABB49303; PMT9312_0242.
KEGGipmi:PMT9312_0242.
PATRICi23004106. VBIProMar70153_0247.

Phylogenomic databases

eggNOGiENOG4107YD0. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000237936.
KOiK03439.
OMAiFERIYIK.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_PROM9
AccessioniPrimary (citable) accession number: Q31CU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.