Q31CR8 (ISPH_PROM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	PMT9312_0266

Organism

Prochlorococcus marinus (strain MIT 9312) [Complete proteome] [HAMAP]

Taxonomic identifier

74546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021154

Sequences

	Sequence		Length	Mass (Da)
	Q31CR8 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 836CF3EE22A9B116	FASTA	398	44,869
	10 20 30 40 50 60 MDTQAFRRSL HHSDRYNRRG FDSPTKRAQA LEEAYQSDLI SSIRDNGFTY NKGRLNIKLA 70 80 90 100 110 120 KAFGFCWGVE RAVAMAYETR RHYPNENIWI TNEIIHNPSV NDHLRKMNVK FISAKNGIKD 130 140 150 160 170 180 FSLVSNGDVV ILPAFGATVQ EMKLLHEKGC HIIDTTCPWV SKVWHTVEKH KKHVFTSIIH 190 200 210 220 230 240 GKFKHEETLA TSSFAGKYLV VLDLEEANYI SEYILGRGNR NEFMNKFAKA CSDGFDPDKD 250 260 270 280 290 300 LDRVGVANQT TMLKSETEEI GKVFEKTMLK KFGPENLNSH FLAFNTICDA TEERQDAMFS 310 320 330 340 350 360 LVDEDLDILV VIGGFNSSNT THLQEIAITK NITSFHIDTP ERISVEENSI LHKPLGLDLE 370 380 390 LKNNFLPSGN INVGITSGAS TPDKVVADVI EKLTVIAS

References

[1]

"Complete sequence of Prochlorococcus marinus str. MIT 9312." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9312.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000111 Genomic DNA. Translation: ABB49327.1.
RefSeq	YP_396763.1. NC_007577.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q31CR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3765053.
GenomeReviews	Gene locus PMT9312_0266 in contig CP000111_GR.
KEGG	pmi:PMT9312_0266.
PATRIC	23004156. VBIProMar70153_0271.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	Q31CR8.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	PMAR74546:PMT9312_0266-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PROM9

Accession

Primary (citable) accession number: Q31CR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program