Reviewed, UniProtKB/Swiss-Prot Q31C72 (UCRI_PROM9)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
      Short name=ISP
      Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	PMT9312_0462

Organism

Prochlorococcus marinus (strain MIT 9312) [Complete proteome] [HAMAP]

Taxonomic identifier

74546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin. HAMAP MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Cytochrome b6-f complex iron-sulfur subunit HAMAP MF_01335		PRO_0000298458
Regions
	Transmembrane	20 – 42	23	Potential
	Domain	65 – 161	97	Rieske
Sites
	Metal binding	107	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	109	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	125	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 127		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q31C72-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 3DE0F53C86564597	FASTA	178	18,924
	10 20 30 40 50 60 MTQLSSNDVP SMGRRQFMNL LTFGTATGVA LGALYPVANY FMPLRAGGGG GGTSAKDELG 70 80 90 100 110 120 NPVTKTGWLA THQAGDRSLV QGLKGDPTYL IVNEGGEIGE FGLNAICTHL GCVVPWDSGA 130 140 150 160 170 NKFICPCHGS QYDTNGKVVR GPAPLSLALA HVDIEDDAVL VKQWSETDFR TNENPWWA

References

[1]

"Complete sequence of Prochlorococcus marinus str. MIT 9312." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000111 Genomic DNA. Translation: ABB49523.1.
RefSeq	YP_396959.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q31C72.
Genome annotation databases
GeneID	3765259.
GenomeReviews	Gene locus PMT9312_0462 in contig CP000111_GR.
KEGG	pmi:PMT9312_0462.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q31C72.
OMA	YPVANYF.
Enzyme and pathway databases
BioCyc	PMAR74546:PMT9312_0462-MON.
Family and domain databases
HAMAP	MF_01335. [Tree]
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_PROM9

Accession

Primary (citable) accession number: Q31C72

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)