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Q31AX4 (SYL_PROM9) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PMT9312_0911
OrganismProchlorococcus marinus (strain MIT 9312) [Complete proteome] [HAMAP]
Taxonomic identifier74546 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 856856Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009394

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q31AX4 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 0ADE75F2D85B2663

FASTA85698,666
        10         20         30         40         50         60 
MISPDKQYET DTNLYNPSEI EKKWQSIWTE NNLYKTDELT ENSDKFYALS MFPYPSGNLH 

        70         80         90        100        110        120 
MGHVRNYVIT DLIARFQRFK GKSVLHPMGW DAFGLPAENA AIERGISPSV WTKKNISHMK 

       130        140        150        160        170        180 
SQLKLLGLSV DWDREFATCD ENYYIWTQYL FLELYKAGLV YQKESEVNWD PIDNTVLANE 

       190        200        210        220        230        240 
QVDSEGKSWR SGALVEKKLL KQWFLRITNY ADELLKDLEK LDNWPERVKI MQDNWIGKSI 

       250        260        270        280        290        300 
GTNINFNINT NPEKKITVFT TRPDTLFGVT YLAISINHSL IKNISDQETI QDIENLKQYL 

       310        320        330        340        350        360 
KNNKNNELEK IGIKTSLIAI NPVNSEPIPI WVASYVLDEY GTGAVMGVPA HDLRDFEFAK 

       370        380        390        400        410        420 
KNNIDIKHVI IKDKSEKTKE LDEAYVENGY LINSNHFNGI ANTIAKLKIS EEGVNNGWAE 

       430        440        450        460        470        480 
NKIQYRLRDW LISRQRYWGC PIPIVNCKKC GSVPLNQSEL PVALPKDIDI SANKINALGD 

       490        500        510        520        530        540 
NYNWINTTCP KCGIAAKKET DTMDTFMCSS WYFLRYPSSR CSTKPFEKIE INNWLPVDQY 

       550        560        570        580        590        600 
VGGVEHAILH LLYARFFTKA LRDNELFEID EPFKKLLTQG MVQSAAYKNN KTGKYISPSD 

       610        620        630        640        650        660 
INDLSNPTDP IDNSKLEVLF EKMSKSKYNG IDPESVIKKY GADTARMFIL FKAPPEKDLE 

       670        680        690        700        710        720 
WGDSDVEGQF RFLSRIWKLY ISCSKDINSK SKSYPNKEKT LIKSMNIAIK EITNDISNNQ 

       730        740        750        760        770        780 
FNTAISELMK FYNSLSNNIN DVNNNLKIDA LKTFCILLAP FAPHISEEIW LLIGFKNSVH 

       790        800        810        820        830        840 
LEHWPSFNAE ALKEDSYVLV IQVNGKVRDK ININNEMNED QIKELTLKRP NILKWTQDKE 

       850 
IRKIIIVKGK IMNIVV 

« Hide

References

[1]"Complete sequence of Prochlorococcus marinus str. MIT 9312."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MIT 9312.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000111 Genomic DNA. Translation: ABB49971.1.
RefSeqYP_397407.1. NC_007577.1.

3D structure databases

ProteinModelPortalQ31AX4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING74546.PMT9312_0911.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB49971; ABB49971; PMT9312_0911.
GeneID3765713.
KEGGpmi:PMT9312_0911.
PATRIC23005582. VBIProMar70153_0968.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPMAR74546:GHRG-933-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PROM9
AccessionPrimary (citable) accession number: Q31AX4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 6, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries