Q318T1 (QUEF_PROM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	PMT9312_1554

Organism

Prochlorococcus marinus (strain MIT 9312) [Complete proteome] [HAMAP]

Taxonomic identifier

74546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 136	136	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000247688

Sequences

	Sequence		Length	Mass (Da)
	Q318T1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 0B656203F7818671	FASTA	136	15,855
	10 20 30 40 50 60 MSTAKLDDST QRPLYGERII EESKIICFEN PNKKRIYEIS IQLPEFTCKC PFSGYPDFAK 70 80 90 100 110 120 LNIIYQPNLS VYELKSLKLY INNFRDIKIS HEEVVNRIMD DLVNEGSPHW IHLNAAFNPR 130 GNVSMQLDIF SGQKRN

References

[1]

"Complete sequence of Prochlorococcus marinus str. MIT 9312." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9312.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000111 Genomic DNA. Translation: ABB50614.1.
RefSeq	YP_398050.1. NC_007577.1.
3D structure databases
ProteinModelPortal	Q318T1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q318T1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3766371.
GenomeReviews	Gene locus PMT9312_1554 in contig CP000111_GR.
KEGG	pmi:PMT9312_1554.
PATRIC	23007120. VBIProMar70153_1723.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG294103.
OMA	FRNAGIF.
PhylomeDB	Q318T1.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	PMAR74546:PMT9312_1554-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_PROM9

Accession

Primary (citable) accession number: Q318T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program