Q318N4 (EFG_PROM9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	PMT9312_1601

Organism

Prochlorococcus marinus (strain MIT 9312) [Complete proteome] [HAMAP]

Taxonomic identifier

74546 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Elongation factor G HAMAP MF_00054_B		PRO_0000263483
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q318N4 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 87D5ACA3B13CD9C7	FASTA	691	75,520
	10 20 30 40 50 60 MARDFPLERV RNIGIAAHID AGKTTTTERI LFYSGVVHKI GEVHDGAAVT DWMAQERERG 70 80 90 100 110 120 ITITAAAIST SWQDHRINII DTPGHVDFTI EVERSMRVLD GVIAVFCAVG GVQPQSETVW 130 140 150 160 170 180 RQADRYSVPR MVFVNKMDRT GADFLKVNNQ IKDRLKANAL PIQLPIGAEG DLTGIIDLVA 190 200 210 220 230 240 NKAYLYKNDL GTDIQEAPIP SEMDDEAAEW RYKLMESVAE NDEELIETFL ETGELSEEQL 250 260 270 280 290 300 KKGIREGVLK HGLVPVLCGS AFKNKGVQLV LDAVVDYLPA PVDVKPIQGV LPSGKEDVRP 310 320 330 340 350 360 SDDNAPFSAL AFKVMSDPYG KLTFVRMYSG VLSKGSYVMN STKDAKERIS RLVILKADER 370 380 390 400 410 420 EEVDELRAGD LGAVLGLKNT TTGDTLCNTE DPIVLETLFI PEPVISVAVE PKTKGDMEKL 430 440 450 460 470 480 SKALTALSEE DPTFRVSTDP ETNQTVIAGM GELHLEILVD RMLREFKVEA NIGAPQVSYR 490 500 510 520 530 540 ETIRSSSKGE GKYARQTGGK GQYGHVIIEM EPAEVGKGFE FVNKIVGGAV PKEYIGPASN 550 560 570 580 590 600 GMKETCESGV LAGYPLIDVK VTLVDGSFHD VDSSEMAFKI AGSMAFKDGV KKCNPVLLEP 610 620 630 640 650 660 MMKVEVESPD DFLGSVIGDL SSRRGQVEGQ SVDDGLSKVQ AKVPLAEMFG YATQLRSMTQ 670 680 690 GRGIFSMEFA NYEEVPRNVA EAIITKNQGN S

References

[1]

"Complete sequence of Prochlorococcus marinus str. MIT 9312." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9312.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000111 Genomic DNA. Translation: ABB50661.1.
RefSeq	YP_398097.1. NC_007577.1.
3D structure databases
HSSP	HSSP built from PDB template 2EFG based on UniProtKB Q5SHN5.
ProteinModelPortal	Q318N4.
SMR	Q318N4. Positions 4-684.
ModBase	Search...
Protein-protein interaction databases
STRING	Q318N4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3766419.
GenomeReviews	Gene locus PMT9312_1601 in contig CP000111_GR.
KEGG	pmi:PMT9312_1601.
PATRIC	23007220. VBIProMar70153_1772.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
PhylomeDB	Q318N4.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	PMAR74546:PMT9312_1601-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_PROM9

Accession

Primary (citable) accession number: Q318N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program