Reviewed, UniProtKB/Swiss-Prot Q313P5 (RIBB_DESDG)

Names and origin

Protein names

Recommended name:
    3,4-dihydroxy-2-butanone 4-phosphate synthase
      Short name=DHBP synthase
    EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	Dde_1050

Organism

Desulfovibrio desulfuricans (strain G20) [Complete proteome] [HAMAP]

Taxonomic identifier

207559 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHBP synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_1000040601
Regions
	Region	37 – 38	2	Substrate binding By similarity
	Region	150 – 154	5	Substrate binding By similarity
Sites
	Metal binding	38	1	Magnesium or manganese 1 By similarity
	Metal binding	38	1	Magnesium or manganese 2 By similarity
	Metal binding	153	1	Magnesium or manganese 2 By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Site	136	1	Essential for catalytic activity By similarity
	Site	174	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q313P5-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 6A33A6DFD25F7585	FASTA	219	23,661
	10 20 30 40 50 60 MSAKESLLTG TFEERVEAAL DALRRGHGVL VTDDEDRENE GDLIFAAETL SDSQMAMLIR 70 80 90 100 110 120 ECSGIVCLCL TDEKVRALGL PMMVENNSSQ YQTAFTVSIE ASCGVSTGVS AADRVCTIQA 130 140 150 160 170 180 AIADDACPSD LCRPGHVFPL RARPGGVLER RGHTEATVDL MRLAGLKPCG VLCEVTNPDG 190 200 210 TMARLPQIVD FGRKHDMVVL TVDDLVRYRE MKENKSAAA

References

[1]

"Complete sequence of Desulfovibrio desulfuricans G20." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goodwin L.A., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000112 Genomic DNA. Translation: ABB37851.1.
RefSeq	YP_387546.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q313P5.
Genome annotation databases
GeneID	3755475.
GenomeReviews	Gene locus Dde_1050 in contig CP000112_GR.
KEGG	dde:Dde_1050.
NMPDR	fig|207559.3.peg.879.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q313P5.
OMA	YGSGIVC.
Enzyme and pathway databases
BioCyc	DDES207559:DDE_1050-MON.
Family and domain databases
HAMAP	MF_00180. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
ProDom	PD003034. DHBP_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_DESDG

Accession

Primary (citable) accession number: Q313P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)