Reviewed, UniProtKB/Swiss-Prot Q311M8 (CHEB1_DESDG)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Dde_1571

Organism

Desulfovibrio desulfuricans (strain G20) [Complete proteome] [HAMAP]

Taxonomic identifier

207559 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225458
Regions
	Domain	3 – 120	118	Response regulatory
	Domain	177 – 369	193	CheB-type methylesterase
Sites
	Active site	189	1	By similarity
	Active site	216	1	By similarity
	Active site	312	1	By similarity
Amino acid modifications
	Modified residue	54	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q311M8-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: E5661FA43AF8805F	FASTA	369	38,865
	10 20 30 40 50 60 MIKVVVVDDS AFMRKALSSM LEKDPGISVV ATARNGEEGL EQIRKHDPDV VTLDIEMPRM 70 80 90 100 110 120 DGLTALRHIM MEMPRPVLMV SSLTVEGAEA TLKAMELGAV DFIPKQLSTV SLDIVKIEKD 130 140 150 160 170 180 LQEKVKAISR RRVPGRSFRP APAVRPAAPA ALRATPRPSA APSSAASSTG TLQVAGGKPV 190 200 210 220 230 240 RDVVAIGVST GGPPAVQKVL SRLPQDFPVG IVIAQHMPAA FTGPFAKRLD GVCAIAVKEA 250 260 270 280 290 300 EDGEMLMPGK AYIAPGGKHV RVRNTRGRLT LEVSAEPAEA LYKPSANELM ESAGLALGRR 310 320 330 340 350 360 SLGVILTGMG SDGLEGIKVL KQKGGHALAQ SDSTCVVYGM PKAIVDAGLA DSVVDIDDMA QAIMNAVYK

References

[1]

"Complete sequence of Desulfovibrio desulfuricans G20." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goodwin L.A., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000112 Genomic DNA. Translation: ABB38368.1.
RefSeq	YP_388063.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q311M8.
Genome annotation databases
GeneID	3755973.
GenomeReviews	Gene locus Dde_1571 in contig CP000112_GR.
KEGG	dde:Dde_1571.
NMPDR	fig|207559.3.peg.975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q311M8.
OMA	RFADISG.
Enzyme and pathway databases
BioCyc	DDES207559:DDE_1571-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_DESDG

Accession

Primary (citable) accession number: Q311M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)