Reviewed, UniProtKB/Swiss-Prot Q30ZJ5 (CHEB2_DESDG)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 2
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	Dde_2104

Organism

Desulfovibrio desulfuricans (strain G20) [Complete proteome] [HAMAP]

Taxonomic identifier

207559 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000225459
Regions
	Domain	6 – 123	118	Response regulatory
	Domain	165 – 356	192	CheB-type methylesterase
Sites
	Active site	177	1	By similarity
	Active site	203	1	By similarity
	Active site	299	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q30ZJ5-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 2FE75F6D0C172C0A	FASTA	356	38,050
	10 20 30 40 50 60 MARHIKVLIV DDSALVRQTL TDIFSADPEL EVVGTASDPF VAVKRMETVV PDVILLDVAM 70 80 90 100 110 120 PRMDGLTFLR KIMSQHPIPV VICSAVTEQG AEASFKAMEY GAVEIIQKPK VSTKQFLEES 130 140 150 160 170 180 SIRICDAVKA AARAQLRKLA AKRFEVTPKL SADAMLPANA GRSVVQRTEK VVVVGASTGG 190 200 210 220 230 240 TEALRVLLEA MPPDCPPIAI VQHMPEHFTA AFAGRLNSTC RIQVKEASDG DVMQRGQALI 250 260 270 280 290 300 APGNLHLLLK RSGSRYYVET KEGPLVRRHR PSVDVLFRSA ARYAGNNAVA AIMTGMGDDG 310 320 330 340 350 AAGMKELHET GAYTIAQDEA TCVVYGMPHE AVKLGGVDAV LPLGALAAAI VKACNS

References

[1]

"Complete sequence of Desulfovibrio desulfuricans G20." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goodwin L.A., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000112 Genomic DNA. Translation: ABB38901.1.
RefSeq	YP_388596.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q30ZJ5.
Genome annotation databases
GeneID	3757112.
GenomeReviews	Gene locus Dde_2104 in contig CP000112_GR.
KEGG	dde:Dde_2104.
NMPDR	fig|207559.3.peg.2064.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q30ZJ5.
OMA	VMCSSLT.
Enzyme and pathway databases
BioCyc	DDES207559:DDE_2104-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_DESDG

Accession

Primary (citable) accession number: Q30ZJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)