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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei565Aminoacyl-adenylateUniRule annotation1
Binding sitei609ATPUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi925ZincUniRule annotation1
Metal bindingi928ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDALA207559:G1G52-2012-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Dde_2142
OrganismiDesulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20))
Taxonomic identifieri207559 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
Proteomesi
  • UP000002710 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000708851 – 934Isoleucine--tRNA ligaseAdd BLAST934

Proteomic databases

PRIDEiQ30ZF7

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi207559.Dde_2142

Structurei

3D structure databases

ProteinModelPortaliQ30ZF7
SMRiQ30ZF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi606 – 610"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q30ZF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKKTLHL PVTKFPMKAN LTQKEPEMLK RWEQTDAYRI MYSTGATKEP
60 70 80 90 100
FVLHDGPPYA NGNIHLGHAL NKIIKDIIIK SRNMQGHPSQ YVPGWDCHGL
110 120 130 140 150
PIEHKVEQQL GEKKKTLPAH AVRRECRKYA TKYLDIQRKE FKRLGVMGQW
160 170 180 190 200
DNPYLTMTPA YEAATARELV RFMEKGSVVR SKKPIYWCCS CETALAEAEV
210 220 230 240 250
EYHDHTSPSI FVRFPMPDAR VAEILGTDPA STSIVIWTTT PWTIPDNMAV
260 270 280 290 300
AVHPEFEYAV VKNDGQHYVL AAELAASCAE TFGWENPQTV RTVTGAALEG
310 320 330 340 350
LVARHPIYNR ESPVVLADYV TLDSGTGCVH TAPGHGREDY ETGLRYGLEI
360 370 380 390 400
YSPMNDEGRF LDSVEFFAGL NVFEANPKVI EKLAELGNLL AQAKIKHSYP
410 420 430 440 450
HCWRCKQPVI FRATTQWFIS MEANGLREKA LKAIRNDVDW IPAWGEERIY
460 470 480 490 500
SMVENRPDWC ISRQRLWGVP IIALLCESCD EAWYDTEWAA GIVEKFANHP
510 520 530 540 550
TGCDYWFETD LSQIVPEGLV CPKCGGTHWA KVKDILDVWF DSGTSFAAVV
560 570 580 590 600
EQREECSFPA DMYLEGSDQH RGWFHSSLLA SVGTRGIPPY KAVLTHGYVV
610 620 630 640 650
DGEGRKMSKS IGNGIAPQEI IDKYGAEILR MWVSSADYRE DVRISEEILS
660 670 680 690 700
RLVDAYRRIR NTCRYLLGNL SDLSAADLLP VGQLESLDRF ALDIAMRAHK
710 720 730 740 750
RVQAAYNDYE FHKVYHTLHN LCVSDLSAFY LDILKDRLYS SKADSKERRS
760 770 780 790 800
AQTAVYHILM LLIRDMAPVL SFTAEEVYSY IPEALRPEAV TVFALREDDI
810 820 830 840 850
PPMEMPAAER EDWEAFLAVR AEVTKAMEPV RKSGEIGHPL DAHVTLFMDD
860 870 880 890 900
ALAAKLKATG ADLRACFIVS KVTVAPLAQA PEQAYTSEEL NGLRISIAKA
910 920 930
PGQKCERCWI WSEELGSSSE HPSLCPRCTG VLCA
Length:934
Mass (Da):105,351
Last modified:December 6, 2005 - v1
Checksum:i09591B35E2AE7F5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000112 Genomic DNA Translation: ABB38939.1
RefSeqiWP_011368039.1, NC_007519.1

Genome annotation databases

EnsemblBacteriaiABB38939; ABB38939; Dde_2142
KEGGidde:Dde_2142

Similar proteinsi

Entry informationi

Entry nameiSYI_DESAG
AccessioniPrimary (citable) accession number: Q30ZF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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