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Q30ZF7

- SYI_DESAG

UniProt

Q30ZF7 - SYI_DESAG

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Dde_2142
Organism
Desulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylate By similarity
Binding sitei609 – 6091ATP By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDALA207559:GH1L-1916-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Dde_2142
OrganismiDesulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20))
Taxonomic identifieri207559 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
ProteomesiUP000002710: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934Isoleucine--tRNA ligaseUniRule annotation
PRO_1000070885Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi207559.Dde_2142.

Structurei

3D structure databases

ProteinModelPortaliQ30ZF7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi606 – 6105"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q30ZF7-1 [UniParc]FASTAAdd to Basket

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MSDYKKTLHL PVTKFPMKAN LTQKEPEMLK RWEQTDAYRI MYSTGATKEP    50
FVLHDGPPYA NGNIHLGHAL NKIIKDIIIK SRNMQGHPSQ YVPGWDCHGL 100
PIEHKVEQQL GEKKKTLPAH AVRRECRKYA TKYLDIQRKE FKRLGVMGQW 150
DNPYLTMTPA YEAATARELV RFMEKGSVVR SKKPIYWCCS CETALAEAEV 200
EYHDHTSPSI FVRFPMPDAR VAEILGTDPA STSIVIWTTT PWTIPDNMAV 250
AVHPEFEYAV VKNDGQHYVL AAELAASCAE TFGWENPQTV RTVTGAALEG 300
LVARHPIYNR ESPVVLADYV TLDSGTGCVH TAPGHGREDY ETGLRYGLEI 350
YSPMNDEGRF LDSVEFFAGL NVFEANPKVI EKLAELGNLL AQAKIKHSYP 400
HCWRCKQPVI FRATTQWFIS MEANGLREKA LKAIRNDVDW IPAWGEERIY 450
SMVENRPDWC ISRQRLWGVP IIALLCESCD EAWYDTEWAA GIVEKFANHP 500
TGCDYWFETD LSQIVPEGLV CPKCGGTHWA KVKDILDVWF DSGTSFAAVV 550
EQREECSFPA DMYLEGSDQH RGWFHSSLLA SVGTRGIPPY KAVLTHGYVV 600
DGEGRKMSKS IGNGIAPQEI IDKYGAEILR MWVSSADYRE DVRISEEILS 650
RLVDAYRRIR NTCRYLLGNL SDLSAADLLP VGQLESLDRF ALDIAMRAHK 700
RVQAAYNDYE FHKVYHTLHN LCVSDLSAFY LDILKDRLYS SKADSKERRS 750
AQTAVYHILM LLIRDMAPVL SFTAEEVYSY IPEALRPEAV TVFALREDDI 800
PPMEMPAAER EDWEAFLAVR AEVTKAMEPV RKSGEIGHPL DAHVTLFMDD 850
ALAAKLKATG ADLRACFIVS KVTVAPLAQA PEQAYTSEEL NGLRISIAKA 900
PGQKCERCWI WSEELGSSSE HPSLCPRCTG VLCA 934
Length:934
Mass (Da):105,351
Last modified:December 6, 2005 - v1
Checksum:i09591B35E2AE7F5F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000112 Genomic DNA. Translation: ABB38939.1.
RefSeqiWP_011368039.1. NC_007519.1.
YP_388634.1. NC_007519.1.

Genome annotation databases

EnsemblBacteriaiABB38939; ABB38939; Dde_2142.
GeneIDi3757151.
KEGGidde:Dde_2142.
PATRICi21743215. VBIDesDes50040_2117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000112 Genomic DNA. Translation: ABB38939.1 .
RefSeqi WP_011368039.1. NC_007519.1.
YP_388634.1. NC_007519.1.

3D structure databases

ProteinModelPortali Q30ZF7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 207559.Dde_2142.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB38939 ; ABB38939 ; Dde_2142 .
GeneIDi 3757151.
KEGGi dde:Dde_2142.
PATRICi 21743215. VBIDesDes50040_2117.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DALA207559:GH1L-1916-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G20.

Entry informationi

Entry nameiSYI_DESAG
AccessioniPrimary (citable) accession number: Q30ZF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 6, 2005
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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