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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
Binding sitei609 – 6091ATPUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDALA207559:GH1L-1916-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Dde_2142
OrganismiDesulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20))
Taxonomic identifieri207559 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
ProteomesiUP000002710 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934Isoleucine--tRNA ligasePRO_1000070885Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi207559.Dde_2142.

Structurei

3D structure databases

ProteinModelPortaliQ30ZF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q30ZF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKKTLHL PVTKFPMKAN LTQKEPEMLK RWEQTDAYRI MYSTGATKEP
60 70 80 90 100
FVLHDGPPYA NGNIHLGHAL NKIIKDIIIK SRNMQGHPSQ YVPGWDCHGL
110 120 130 140 150
PIEHKVEQQL GEKKKTLPAH AVRRECRKYA TKYLDIQRKE FKRLGVMGQW
160 170 180 190 200
DNPYLTMTPA YEAATARELV RFMEKGSVVR SKKPIYWCCS CETALAEAEV
210 220 230 240 250
EYHDHTSPSI FVRFPMPDAR VAEILGTDPA STSIVIWTTT PWTIPDNMAV
260 270 280 290 300
AVHPEFEYAV VKNDGQHYVL AAELAASCAE TFGWENPQTV RTVTGAALEG
310 320 330 340 350
LVARHPIYNR ESPVVLADYV TLDSGTGCVH TAPGHGREDY ETGLRYGLEI
360 370 380 390 400
YSPMNDEGRF LDSVEFFAGL NVFEANPKVI EKLAELGNLL AQAKIKHSYP
410 420 430 440 450
HCWRCKQPVI FRATTQWFIS MEANGLREKA LKAIRNDVDW IPAWGEERIY
460 470 480 490 500
SMVENRPDWC ISRQRLWGVP IIALLCESCD EAWYDTEWAA GIVEKFANHP
510 520 530 540 550
TGCDYWFETD LSQIVPEGLV CPKCGGTHWA KVKDILDVWF DSGTSFAAVV
560 570 580 590 600
EQREECSFPA DMYLEGSDQH RGWFHSSLLA SVGTRGIPPY KAVLTHGYVV
610 620 630 640 650
DGEGRKMSKS IGNGIAPQEI IDKYGAEILR MWVSSADYRE DVRISEEILS
660 670 680 690 700
RLVDAYRRIR NTCRYLLGNL SDLSAADLLP VGQLESLDRF ALDIAMRAHK
710 720 730 740 750
RVQAAYNDYE FHKVYHTLHN LCVSDLSAFY LDILKDRLYS SKADSKERRS
760 770 780 790 800
AQTAVYHILM LLIRDMAPVL SFTAEEVYSY IPEALRPEAV TVFALREDDI
810 820 830 840 850
PPMEMPAAER EDWEAFLAVR AEVTKAMEPV RKSGEIGHPL DAHVTLFMDD
860 870 880 890 900
ALAAKLKATG ADLRACFIVS KVTVAPLAQA PEQAYTSEEL NGLRISIAKA
910 920 930
PGQKCERCWI WSEELGSSSE HPSLCPRCTG VLCA
Length:934
Mass (Da):105,351
Last modified:December 6, 2005 - v1
Checksum:i09591B35E2AE7F5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000112 Genomic DNA. Translation: ABB38939.1.
RefSeqiWP_011368039.1. NC_007519.1.
YP_388634.1. NC_007519.1.

Genome annotation databases

EnsemblBacteriaiABB38939; ABB38939; Dde_2142.
KEGGidde:Dde_2142.
PATRICi21743215. VBIDesDes50040_2117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000112 Genomic DNA. Translation: ABB38939.1.
RefSeqiWP_011368039.1. NC_007519.1.
YP_388634.1. NC_007519.1.

3D structure databases

ProteinModelPortaliQ30ZF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi207559.Dde_2142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB38939; ABB38939; Dde_2142.
KEGGidde:Dde_2142.
PATRICi21743215. VBIDesDes50040_2117.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciDALA207559:GH1L-1916-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G20.

Entry informationi

Entry nameiSYI_DESAG
AccessioniPrimary (citable) accession number: Q30ZF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 6, 2005
Last modified: May 27, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.