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Q30Z14 (GLGB_DESAG) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Dde_2285
OrganismDesulfovibrio alaskensis (strain G20) (Desulfovibrio desulfuricans (strain G20)) [Complete proteome] [HAMAP]
Taxonomic identifier207559 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6386381,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260652

Sites

Active site3201Nucleophile By similarity
Active site3731Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q30Z14 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 2A94D18BA9E6556C

FASTA63872,878
        10         20         30         40         50         60 
MATPVESGTV PCSLEPFDVY LFGRGEHWDI YRVLGAHAHA QDGETGYRFA VWAPNAHAVS 

        70         80         90        100        110        120 
LVGPFNDWRS GDFPLFPVGT SGIWAGFVAG MEYGRLYKFA VQGADGTVRL KTDPYALYCE 

       130        140        150        160        170        180 
MRPGTASFTW SLDSYAWNDA AWMQRRREAG PPLQQPVSIY ELHAGSWMRR HGEGHPFVNW 

       190        200        210        220        230        240 
DELAGTLIPY VRDAGFTHIE LMPVAEHPLD QSWGYQTGAY YAPTSRFGTP DDLRRFVDLC 

       250        260        270        280        290        300 
HQQGIGVILD WVPAHFPKDD WSLGRFDGTA LYEHLDPRLG EHPDWGTYVF NYGRHEVRNF 

       310        320        330        340        350        360 
LFANALYWFK EFHVDGLRID AVASMLYLDY SRKEGEWLPN VHGGNENLEA IDFLRELNRV 

       370        380        390        400        410        420 
VHEQYPGVMM IAEESTSWPG VSRPLYTGGL GFTFKWNMGW MHDTLNYMRQ DPVFRAYQHS 

       430        440        450        460        470        480 
SLTFSMLYAF SENFVLPLSH DEVVHGKGAL LSKMPGDMWQ QQANLRLMYA YMWAHPGKKL 

       490        500        510        520        530        540 
LFMGGEIGQW NEWSESREPD WCLREFPAHE GIRNLVRDLN GIYAQEPAMH RHDHDWSGFR 

       550        560        570        580        590        600 
WLDFSDYGCS VISFARFAED SPPVMWVFNF TPVVRRWYRV PCPRAGEWQE VLNTDSGYYG 

       610        620        630 
GSNVGNGGGA VACTDNWHGG HFMELTLPPL AAVALKPV 

« Hide

References

[1]"Complete genome sequence and updated annotation of Desulfovibrio alaskensis G20."
Hauser L.J., Land M.L., Brown S.D., Larimer F., Keller K.L., Rapp-Giles B.J., Price M.N., Lin M., Bruce D.C., Detter J.C., Tapia R., Han C.S., Goodwin L.A., Cheng J.F., Pitluck S., Copeland A., Lucas S., Nolan M. expand/collapse author list , Lapidus A.L., Palumbo A.V., Wall J.D.
J. Bacteriol. 193:4268-4269(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000112 Genomic DNA. Translation: ABB39082.1.
RefSeqYP_388777.1. NC_007519.1.

3D structure databases

ProteinModelPortalQ30Z14.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING207559.Dde_2285.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB39082; ABB39082; Dde_2285.
GeneID3757296.
KEGGdde:Dde_2285.
PATRIC21743499. VBIDesDes50040_2257.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAALYWFKE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycDALA207559:GH1L-2047-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_DESAG
AccessionPrimary (citable) accession number: Q30Z14
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries