Reviewed, UniProtKB/Swiss-Prot Q30WL8 (SAHH_DESDG)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	Dde_3134

Organism

Desulfovibrio desulfuricans (strain G20) [Complete proteome] [HAMAP]

Taxonomic identifier

207559 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024724
Regions
	Region	230 – 398	169	NAD binding By similarity
Sites
	Binding site	66	1	Substrate By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity
	Binding site	237	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q30WL8-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: F2149193D439D172	FASTA	479	52,811
	10 20 30 40 50 60 MTDARKALPL DLSLENKVKD LSQADFGYKE MQLSEREMPG LMELIAKHGA EKPLKGLKVT 70 80 90 100 110 120 GSLHMTIQTA MLIKTLYELG ADIRWASCNI FSTQDHAAAA IADSGMAKVF AWKGETLEEY 130 140 150 160 170 180 WWCTEMALTW PDGSGPDLIV DDGGDATMLI HKGVEAEKNP ALLEKSYDNK EFQIVMNRIA 190 200 210 220 230 240 LSMKNDPGKW TRVAAKVRGV SEETTTGVHR LYHMEKDGSL LFPAINVNDS VTKSKFDNLY 250 260 270 280 290 300 GCRESLADGI KRATDVMIAG KTVVVLGYGD VGKGCAHSMR GFGARVLVTE IDPICALQAA 310 320 330 340 350 360 MEGFEVVTMD EAAPEGDIFV TATGNFKVIT GEHMERMKDE AIVCNIGHFD NEIDMAYLED 370 380 390 400 410 420 STECSCLNIK PQVDKWTLKS GRSIIVLAEG RLVNLGCATG HPSFVMSNSF TNQVLAQLEL 430 440 450 460 470 ASNPDLERKV YILPKKLDEE VARLHLARLG AKLTTLTDEQ AEYIGVDKTG PFKPDHYRY

References

[1]

"Complete sequence of Desulfovibrio desulfuricans G20." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goodwin L.A., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000112 Genomic DNA. Translation: ABB39928.1.
RefSeq	YP_389623.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q30WL8.
Genome annotation databases
GeneID	3758108.
GenomeReviews	Gene locus Dde_3134 in contig CP000112_GR.
KEGG	dde:Dde_3134.
NMPDR	fig|207559.3.peg.3734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q30WL8.
OMA	HMRAMKD.
Enzyme and pathway databases
BioCyc	DDES207559:DDE_3134-MON.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_DESDG

Accession

Primary (citable) accession number: Q30WL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)