Reviewed, UniProtKB/Swiss-Prot Q30U81 (BIOB_SULDN)

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	Suden_0169

Organism

Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251)) [Complete proteome] [HAMAP]

Taxonomic identifier

326298 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Sulfurimonas

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP MF_01694 Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity. HAMAP MF_01694
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP MF_01694
Subunit structure	Homodimer By similarity. HAMAP MF_01694
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Biotin synthase HAMAP MF_01694		PRO_0000381668
Sites
	Metal binding	18	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	22	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	25	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	62	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	97	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	223	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q30U81-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: B24959A6F62F9709	FASTA	281	31,152
	10 20 30 40 50 60 MNQKIFLCSI CNINSGTCNE DCKFCSQSVR YKADIDRYKQ KEISLILKEA QDAYSNGALG 70 80 90 100 110 120 FCLVTSDKGL NDKTLNFVCS IAEVLSKELP ELRLIACNGT ATIEQLLTLR ASGIKAYNHN 130 140 150 160 170 180 LETSREFYPK ICTTHSWDER YETCQNINNA GLVLISGGIF GLGEREEDRV SMLKSLDSLK 190 200 210 220 230 240 PTSVPINFYH HNEALELSPN PLSVDEALSL IKLTRAMIPD AQRIMVAGGR ELMFGDRESE 250 260 270 280 IFANGANSIV IGNYLTTSGK AMQRDLEMLK SLNLDVASSV K

References

[1]

"Genome of the epsilonproteobacterial chemolithoautotroph Sulfurimonas denitrificans." Sievert S.M., Scott K.M., Klotz M.G., Chain P.S.G., Hauser L.J., Hemp J., Huegler M., Land M., Lapidus A., Larimer F.W., Lucas S., Malfatti S.A., Meyer F., Paulsen I.T., Ren Q., Simon J., Bailey K., Diaz E. , Fitzpatrick K.A., Glover B., Gwatney N., Korajkic A., Long A., Mobberley J.M., Pantry S.N., Pazder G., Peterson S., Quintanilla J.D., Sprinkle R., Stephens J., Thomas P., Vaughn R., Weber M.J., Wooten L.L. Appl. Environ. Microbiol. 74:1145-1156(2008) [PubMed: 18065616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000153 Genomic DNA. Translation: ABB43450.1.
RefSeq	YP_392685.1.
3D structure databases
SMR	Q30U81. Positions 3-276.
ModBase	Search...
Protein-protein interaction databases
STRING	Q30U81.
Genome annotation databases
GeneID	3763119.
GenomeReviews	Gene locus Suden_0169 in contig CP000153_GR.
KEGG	tdn:Suden_0169.
NMPDR	fig|326298.3.peg.105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HBG405569.
OMA	AGLEVCS.
ProtClustDB	PRK08508.
Enzyme and pathway databases
BioCyc	TDEN326298:TMDEN_0169-MONOMER.
Family and domain databases
HAMAP	MF_01694. BioB. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR006638. Elp3/MiaB/NifB. IPR007197. Radical_SAM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIOB_SULDN

Accession

Primary (citable) accession number: Q30U81

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	December 6, 2005
Last modified:	March 2, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)