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Q30U81 (BIOB_SULDN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281Biotin synthase HAMAP-Rule MF_01694
PRO_0000381668

Sites

Metal binding181Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding221Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding251Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding621Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding971Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2231Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q30U81 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: B24959A6F62F9709

FASTA28131,152
        10         20         30         40         50         60 
MNQKIFLCSI CNINSGTCNE DCKFCSQSVR YKADIDRYKQ KEISLILKEA QDAYSNGALG 

        70         80         90        100        110        120 
FCLVTSDKGL NDKTLNFVCS IAEVLSKELP ELRLIACNGT ATIEQLLTLR ASGIKAYNHN 

       130        140        150        160        170        180 
LETSREFYPK ICTTHSWDER YETCQNINNA GLVLISGGIF GLGEREEDRV SMLKSLDSLK 

       190        200        210        220        230        240 
PTSVPINFYH HNEALELSPN PLSVDEALSL IKLTRAMIPD AQRIMVAGGR ELMFGDRESE 

       250        260        270        280 
IFANGANSIV IGNYLTTSGK AMQRDLEMLK SLNLDVASSV K 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000153 Genomic DNA. Translation: ABB43450.1.
RefSeqYP_392685.1. NC_007575.1.

3D structure databases

ProteinModelPortalQ30U81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING326298.Suden_0169.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB43450; ABB43450; Suden_0169.
GeneID3763119.
KEGGtdn:Suden_0169.
PATRIC23768804. VBISulDen68967_0175.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK08508.

Enzyme and pathway databases

BioCycSDEN326298:GH9P-174-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SULDN
AccessionPrimary (citable) accession number: Q30U81
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 6, 2005
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways