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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi511 – 5111MagnesiumUniRule annotation
Metal bindingi517 – 5171MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSDEN326298:GH9P-444-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Suden_0428
OrganismiSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Taxonomic identifieri326298 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeSulfurimonas
ProteomesiUP000002714 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Polyribonucleotide nucleotidyltransferasePRO_0000329891Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi326298.Suden_0428.

Structurei

3D structure databases

ProteinModelPortaliQ30TH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini577 – 63761KHUniRule annotationAdd
BLAST
Domaini654 – 72168S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF13014. KH_3. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q30TH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYDIKIDLK NKNEEYSFGE VAKQANGAAW LKSGNTVILA TVVIDETEIV
60 70 80 90 100
KDDFLPLTVQ YIEKAYAAGK IPGGFFKRET KASDFETLTS RIVDRSLRPL
110 120 130 140 150
FPKGFGHPTQ ITIMVFSVDK ESDLQVLALN AASAALYVSD IDINKSVSAI
160 170 180 190 200
RVAKIDGELI LNPTLSELDK STLDLYLSGT KEDLLMIEMR SKGGIKVDTN
210 220 230 240 250
LMIDPLMDPT LSSAIIATHV SNAVPEDELI TILAKAEELL FETNAMYEEM
260 270 280 290 300
FKEFKKETSP LECKAHALNE EMLKYVRSNH YSDIKSAMNQ MAKSERSTAL
310 320 330 340 350
RQLRKKIIIT KEEWDEVELK DVIESVKKEQ VRGQILNDRV RADGRALNEI
360 370 380 390 400
RPISISTNVL PSAHASCLFT RGQTQALVVL TMGGPKDAQM FENLTDEGTQ
410 420 430 440 450
NENFMVHYNF PGFSVGEASP IMGTKRRELG HGNLAKRALE PIVNLEGQTV
460 470 480 490 500
RLVSEILESN GSSSMATVCG GYMALRAADI ETSDTIAGIA MGMVSEGDKY
510 520 530 540 550
AILSDIMGLE DHDGDLDFKV TGSKDGITAM QMDIKLGGIS LNILKEALYQ
560 570 580 590 600
AKEGRAHIIN IMQEAEKNIS FNDGVLPSTD FFHINPSFIG DIIGQAGKTI
610 620 630 640 650
REIIEKFEVA IDIDKKDGKV KVTGKSKSGV QAAREHIEGI VSTPKMPKIE
660 670 680 690 700
YKVGDIHKGI VKKIVEFGAF IELPDGVDGL LHISKISDQR VEKVSDILKE
710 720
GEEINVEILE FKGNKISLGI A
Length:721
Mass (Da):79,428
Last modified:December 6, 2005 - v1
Checksum:iC76693166F5B8581
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000153 Genomic DNA. Translation: ABB43709.1.
RefSeqiWP_011372063.1. NC_007575.1.
YP_392944.1. NC_007575.1.

Genome annotation databases

EnsemblBacteriaiABB43709; ABB43709; Suden_0428.
KEGGitdn:Suden_0428.
PATRICi23769366. VBISulDen68967_0446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000153 Genomic DNA. Translation: ABB43709.1.
RefSeqiWP_011372063.1. NC_007575.1.
YP_392944.1. NC_007575.1.

3D structure databases

ProteinModelPortaliQ30TH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326298.Suden_0428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB43709; ABB43709; Suden_0428.
KEGGitdn:Suden_0428.
PATRICi23769366. VBISulDen68967_0446.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciSDEN326298:GH9P-444-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF13014. KH_3. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33889 / DSM 1251.

Entry informationi

Entry nameiPNP_SULDN
AccessioniPrimary (citable) accession number: Q30TH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 6, 2005
Last modified: May 27, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.