Q30TE6 (TRMD_SULDN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Suden_0454

Organism

Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251)) [Complete proteome] [HAMAP]

Taxonomic identifier

326298 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Sulfurimonas

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000257489
Regions
	Region	132 – 137	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	112	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q30TE6 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: D1C1697494821502	FASTA	231	26,047
	10 20 30 40 50 60 MKFTFVTLFQ NIVEGYFNDS ILKRAIQKEI LEVEYLNPRD FSDSRHNKVD DSAVGGGAGM 70 80 90 100 110 120 VMNPQPLFDA LDELKRVDSE VHILFLTPVA KSFKQNDAKR LSKKPHIAFV SGRYEGIDER 130 140 150 160 170 180 VIEKYADEVF SIGDYILTGG ELPSLVICDA ISRNVDGVLG NSDSLSVESF ETELLEAPSF 190 200 210 220 230 SKPEIYKNIS VPSEYLKGNH SKIRSLKLAL STCKTKFFRP EQLLKHKTQL H

References

[1]

"Genome of the epsilonproteobacterial chemolithoautotroph Sulfurimonas denitrificans." Sievert S.M., Scott K.M., Klotz M.G., Chain P.S.G., Hauser L.J., Hemp J., Huegler M., Land M., Lapidus A., Larimer F.W., Lucas S., Malfatti S.A., Meyer F., Paulsen I.T., Ren Q., Simon J., Bailey K., Diaz E. , Fitzpatrick K.A., Glover B., Gwatney N., Korajkic A., Long A., Mobberley J.M., Pantry S.N., Pazder G., Peterson S., Quintanilla J.D., Sprinkle R., Stephens J., Thomas P., Vaughn R., Weber M.J., Wooten L.L. Appl. Environ. Microbiol. 74:1145-1156(2008) [PubMed: 18065616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33889 / DSM 1251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000153 Genomic DNA. Translation: ABB43735.1.
RefSeq	YP_392970.1. NC_007575.1.
3D structure databases
ProteinModelPortal	Q30TE6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q30TE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3764667.
GenomeReviews	Gene locus Suden_0454 in contig CP000153_GR.
KEGG	tdn:Suden_0454.
NMPDR	fig|326298.3.peg.1644.
PATRIC	23769420. VBISulDen68967_0472.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	RYLYDRE.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	TDEN326298:TMDEN_0454-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_SULDN

Accession

Primary (citable) accession number: Q30TE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program