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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Active sitei128Proton acceptorUniRule annotation1
Binding sitei155Allosteric activator ADPUniRule annotation1
Binding sitei163Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei223SubstrateUniRule annotation1
Binding sitei245Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSDEN326298:G1G5D-577-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Suden_0549
OrganismiSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Taxonomic identifieri326298 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeSulfurimonas
Proteomesi
  • UP000002714 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000720581 – 319ATP-dependent 6-phosphofructokinaseAdd BLAST319

Proteomic databases

PRIDEiQ30T53

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi326298.Suden_0549

Structurei

3D structure databases

ProteinModelPortaliQ30T53
SMRiQ30T53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 128Substrate bindingUniRule annotation3
Regioni170 – 172Substrate bindingUniRule annotation3
Regioni186 – 188Allosteric activator ADP bindingUniRule annotation3
Regioni251 – 254Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiGKLHSII
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q30T53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIAILSSG GDVSGMNPAI KHFVEYALHN GLTPYFVYNG FEGLIDNKIT
60 70 80 90 100
KASHSDVAGI INRGGTILGS SRSKRFMLKN YREVAKKNLD ALDIDMLIVL
110 120 130 140 150
GGDGSFRGMD IFYKEHGVKF CGIPSTIDND INGTIYCLGV DTALNVIKDS
160 170 180 190 200
IDNIRDTASS FSRAFVIETM GRDCGYLALV SSLCSGAELC LIPEVEYNLS
210 220 230 240 250
SYEDSFKEQI KNGRKYFIVI VSEGIKDNSK EIAEWLEQKV GIESRVTVLG
260 270 280 290 300
HTQRGGTPSI YDRLMAYKFV NHAIDALLGD INSSVVCYSK TGFIHKDIDE
310
ITSQKYMLDP ELLSYLKKF
Length:319
Mass (Da):35,330
Last modified:December 6, 2005 - v1
Checksum:i0A684E61EF5CFB28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000153 Genomic DNA Translation: ABB43828.1
RefSeqiWP_011372182.1, NC_007575.1

Genome annotation databases

EnsemblBacteriaiABB43828; ABB43828; Suden_0549
KEGGitdn:Suden_0549

Similar proteinsi

Entry informationi

Entry nameiPFKA_SULDN
AccessioniPrimary (citable) accession number: Q30T53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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