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Q30SH2

- SYI_SULDN

UniProt

Q30SH2 - SYI_SULDN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSDEN326298:GH9P-814-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Suden_0780
OrganismiSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Taxonomic identifieri326298 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeSulfurimonas
ProteomesiUP000002714: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000022139Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi326298.Suden_0780.

Structurei

3D structure databases

ProteinModelPortaliQ30SH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q30SH2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTLLLP TTKFEMRGNL INNEPIRYAS WDEKKIYEKM KKNRKNAQSF
60 70 80 90 100
TLHDGPPYAN GHTHIGHALN KILKDIIVKH NYFSGKSVRF TPGWDCHGLP
110 120 130 140 150
IEQQVEKKLG GKQKKELLEK AKIRELCRAH AAEFVDIQRE EFKKLGVIAD
160 170 180 190 200
WENPYVTMDF KFEANIYRTL CNVAKKGLLI ERSKPVFWSW AERTALAEAE
210 220 230 240 250
VEYEDKEDYS IFIAFELSDE AKAKLSINSK AALVIWTTTP WTIPANTGIS
260 270 280 290 300
LNPEEEYILT TTGYIVAKKL LSSLNESKIL SGDIAQTFKA KEFENLLALN
310 320 330 340 350
PLNGRTSRIV LGEHVLVENG TGCVHTAPGH GEDDYRIGLV YDLEVVMPVD
360 370 380 390 400
ETGCYDETIV RDGLIPNAED FLGRHIFKSN EDLITLMGES VVHVSKFKHS
410 420 430 440 450
YPHCWRSHTP LIFRATKQWF ISVDEKPSGS DKTLRDIALS EVEKTLFFPE
460 470 480 490 500
TGRNRLKSMV ANRPDWCISR QRDWGVPIAF FRVKATGEVL LDEKVLNFTA
510 520 530 540 550
MVFEMHGSDA WYSMPTEQLL YPGAGYSADE LEKVTDILDV WFDSGSTWYS
560 570 580 590 600
VLKSRNYDAG EFQADLYVEG SDQHRGWFQS SMFLSAAVEH KAPYKGVLTH
610 620 630 640 650
GFTVDEKGEK MSKSKGNVVA PETVLKEYGS EILRLWVASS DYQGDLKISQ
660 670 680 690 700
SILKQSAENY RKLRNTFRIM LANINDLHAL TPYEKMGELD KWILNEAKSV
710 720 730 740 750
FDGVHKSFSN YNFVNGMSLL NNFIVNELSG IYIDITKDSL YCDAKDDARR
760 770 780 790 800
TSSQSAMALI VKSLLTLIAP ILTYTADEIV EYLPEVIRES KEDIFDFVHK
810 820 830 840 850
NIDVAESGFN TDYMYAAREK FYEIVDGLKK EKIIKNTLEL VIVTESKKIA
860 870 880 890 900
EMDKTAAEDW FVVSGISHSE VIAELGKFEV DGSIFVIQKA TAAKCPRCWK
910
YQAKDETATC TRCSKVLNA
Length:919
Mass (Da):104,455
Last modified:December 6, 2005 - v1
Checksum:i10250E6BCF51E029
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000153 Genomic DNA. Translation: ABB44059.1.
RefSeqiWP_011372412.1. NC_007575.1.
YP_393294.1. NC_007575.1.

Genome annotation databases

EnsemblBacteriaiABB44059; ABB44059; Suden_0780.
GeneIDi3764306.
KEGGitdn:Suden_0780.
PATRICi23770118. VBISulDen68967_0807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000153 Genomic DNA. Translation: ABB44059.1 .
RefSeqi WP_011372412.1. NC_007575.1.
YP_393294.1. NC_007575.1.

3D structure databases

ProteinModelPortali Q30SH2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 326298.Suden_0780.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB44059 ; ABB44059 ; Suden_0780 .
GeneIDi 3764306.
KEGGi tdn:Suden_0780.
PATRICi 23770118. VBISulDen68967_0807.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SDEN326298:GH9P-814-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33889 / DSM 1251.

Entry informationi

Entry nameiSYI_SULDN
AccessioniPrimary (citable) accession number: Q30SH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 6, 2005
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3