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Q30SH2

- SYI_SULDN

UniProt

Q30SH2 - SYI_SULDN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi913 – 9131ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSDEN326298:GH9P-814-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Suden_0780
    OrganismiSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
    Taxonomic identifieri326298 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeSulfurimonas
    ProteomesiUP000002714: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000022139Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi326298.Suden_0780.

    Structurei

    3D structure databases

    ProteinModelPortaliQ30SH2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q30SH2-1 [UniParc]FASTAAdd to Basket

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    MDYKDTLLLP TTKFEMRGNL INNEPIRYAS WDEKKIYEKM KKNRKNAQSF    50
    TLHDGPPYAN GHTHIGHALN KILKDIIVKH NYFSGKSVRF TPGWDCHGLP 100
    IEQQVEKKLG GKQKKELLEK AKIRELCRAH AAEFVDIQRE EFKKLGVIAD 150
    WENPYVTMDF KFEANIYRTL CNVAKKGLLI ERSKPVFWSW AERTALAEAE 200
    VEYEDKEDYS IFIAFELSDE AKAKLSINSK AALVIWTTTP WTIPANTGIS 250
    LNPEEEYILT TTGYIVAKKL LSSLNESKIL SGDIAQTFKA KEFENLLALN 300
    PLNGRTSRIV LGEHVLVENG TGCVHTAPGH GEDDYRIGLV YDLEVVMPVD 350
    ETGCYDETIV RDGLIPNAED FLGRHIFKSN EDLITLMGES VVHVSKFKHS 400
    YPHCWRSHTP LIFRATKQWF ISVDEKPSGS DKTLRDIALS EVEKTLFFPE 450
    TGRNRLKSMV ANRPDWCISR QRDWGVPIAF FRVKATGEVL LDEKVLNFTA 500
    MVFEMHGSDA WYSMPTEQLL YPGAGYSADE LEKVTDILDV WFDSGSTWYS 550
    VLKSRNYDAG EFQADLYVEG SDQHRGWFQS SMFLSAAVEH KAPYKGVLTH 600
    GFTVDEKGEK MSKSKGNVVA PETVLKEYGS EILRLWVASS DYQGDLKISQ 650
    SILKQSAENY RKLRNTFRIM LANINDLHAL TPYEKMGELD KWILNEAKSV 700
    FDGVHKSFSN YNFVNGMSLL NNFIVNELSG IYIDITKDSL YCDAKDDARR 750
    TSSQSAMALI VKSLLTLIAP ILTYTADEIV EYLPEVIRES KEDIFDFVHK 800
    NIDVAESGFN TDYMYAAREK FYEIVDGLKK EKIIKNTLEL VIVTESKKIA 850
    EMDKTAAEDW FVVSGISHSE VIAELGKFEV DGSIFVIQKA TAAKCPRCWK 900
    YQAKDETATC TRCSKVLNA 919
    Length:919
    Mass (Da):104,455
    Last modified:December 6, 2005 - v1
    Checksum:i10250E6BCF51E029
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000153 Genomic DNA. Translation: ABB44059.1.
    RefSeqiYP_393294.1. NC_007575.1.

    Genome annotation databases

    EnsemblBacteriaiABB44059; ABB44059; Suden_0780.
    GeneIDi3764306.
    KEGGitdn:Suden_0780.
    PATRICi23770118. VBISulDen68967_0807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000153 Genomic DNA. Translation: ABB44059.1 .
    RefSeqi YP_393294.1. NC_007575.1.

    3D structure databases

    ProteinModelPortali Q30SH2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 326298.Suden_0780.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB44059 ; ABB44059 ; Suden_0780 .
    GeneIDi 3764306.
    KEGGi tdn:Suden_0780.
    PATRICi 23770118. VBISulDen68967_0807.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SDEN326298:GH9P-814-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33889 / DSM 1251.

    Entry informationi

    Entry nameiSYI_SULDN
    AccessioniPrimary (citable) accession number: Q30SH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3