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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei128S-adenosyl-L-methionineUniRule annotation1
Binding sitei153S-adenosyl-L-methionineUniRule annotation1
Binding sitei180S-adenosyl-L-methionineUniRule annotation1
Binding sitei206SubstrateUniRule annotation1
Binding sitei236SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Suden_0785
OrganismiSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251))
Taxonomic identifieri326298 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeSulfurimonas
Proteomesi
  • UP000002714 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002292081 – 390tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST390

Interactioni

Protein-protein interaction databases

STRINGi326298.Suden_0785.

Structurei

3D structure databases

ProteinModelPortaliQ30SG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q30SG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHLHIAEFK EVEIPSSKDG VSFNFIAKNS SHQEEKLISI TVDEDEFFLL
60 70 80 90 100
AIDENSKKLL KSDKLTRPPS IFNVHKALLS YASATCMNII SSNVPQNQKN
110 120 130 140 150
IHLREVKALK DINYFATTFP KKEKICIEVG FGSGRHLLHQ AQNNPNILYI
160 170 180 190 200
GIEIHTPSIE QLLKQISIKN IENILVLNYD ARLFMELVPS NKIDKIYVHF
210 220 230 240 250
PVPWDKKPHR RVISTAFIEE ARRILNIGGT LELRTDSENY YAYSYETFIA
260 270 280 290 300
FEKTTLHINK NRDIAITSKY EDRWKRMQKN FYDVTMINSE ESPELSLEGD
310 320 330 340 350
FNFSHVKLNT QEVINLYKKT QKFDSGFINF ERSYFLDDGA MLRVSMGNFD
360 370 380 390
KPEHLYVIVK ENKAYYYPKT PLRSKSNLMA HKLLNGVLCE
Length:390
Mass (Da):45,297
Last modified:December 6, 2005 - v1
Checksum:iF8E8801AEFE94201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000153 Genomic DNA. Translation: ABB44064.1.
RefSeqiWP_011372417.1. NC_007575.1.

Genome annotation databases

EnsemblBacteriaiABB44064; ABB44064; Suden_0785.
KEGGitdn:Suden_0785.
PATRICi23770140. VBISulDen68967_0812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000153 Genomic DNA. Translation: ABB44064.1.
RefSeqiWP_011372417.1. NC_007575.1.

3D structure databases

ProteinModelPortaliQ30SG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326298.Suden_0785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB44064; ABB44064; Suden_0785.
KEGGitdn:Suden_0785.
PATRICi23770140. VBISulDen68967_0812.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_SULDN
AccessioniPrimary (citable) accession number: Q30SG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.