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Q30QK9 (DAPE_SULDN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Succinyl-diaminopimelate desuccinylase
Short name=SDAP desuccinylase
EC=3.5.1.18
Alternative name(s):
N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase
Gene names
Name:dapE
Ordered Locus Names:Suden_1445
OrganismSulfurimonas denitrificans (strain ATCC 33889 / DSM 1251) (Thiomicrospira denitrificans (strain ATCC 33889 / DSM 1251)) [Complete proteome] [HAMAP]
Taxonomic identifier326298 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Sulfurimonas ›

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls By similarity. HAMAP-Rule MF_01690

Catalytic activity

N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate. HAMAP-Rule MF_01690

Cofactor

Binds 1 Zn2+ ion per subunit By similarity.

Binds 1 Co2+ ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3. HAMAP-Rule MF_01690

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the peptidase M20A family. DapE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Succinyl-diaminopimelate desuccinylase HAMAP-Rule MF_01690
PRO_0000375759

Sites

Active site661 By similarity
Active site1251Proton acceptor By similarity
Metal binding641Cobalt or zinc 1 By similarity
Metal binding951Cobalt or zinc 1 By similarity
Metal binding951Cobalt or zinc 2 By similarity
Metal binding1261Cobalt or zinc 2 By similarity
Metal binding1541Cobalt or zinc 1 By similarity
Metal binding3391Cobalt or zinc 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q30QK9 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 4E3088A08680BCE5

FASTA36540,660
        10         20         30         40         50         60 
MKTIELFKFM ISAKSQTPDD GGLLDFIQNY LDDFEAIRVD VEGVKNLFLY KKFSQGDHLC 

        70         80         90        100        110        120 
FAGHVDVVPA GDGWDSDPYI ATERDGYIYG RGAQDMKSGV AAFVQAIKDT KHFNGTLSLL 

       130        140        150        160        170        180 
LTSDEEGEGT YGTIEVLNYL RDKSMLPDFA VVAEPTCEMV FGDAIKVGRR GSINGYITLR 

       190        200        210        220        230        240 
GKQGHAAYPE KSINPINLIA PKLANMAGVD LDNGDEFFSP SKFVITDIRA GMQVTNVTPN 

       250        260        270        280        290        300 
ELKMMFNVRN TTLTSQKEIR EFVEKNLEDL DYDLRLTQGS YPFKTDTKTK LVKNIDASIE 

       310        320        330        340        350        360 
QISGIKPKHS TAGGTSDARH MAPLGIDVIE FGVINDTIHA INERTTKDEV KKLYEVFKHL 


IDTWK

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000153 Genomic DNA. Translation: ABB44722.1.
RefSeqYP_393957.1. NC_007575.1.

3D structure databases

ProteinModelPortalQ30QK9.
ModBaseSearch...

Protein-protein interaction databases

STRING326298.Suden_1445.

Protein family/group databases

MEROPSM20.010.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB44722; ABB44722; Suden_1445.
GeneID3763380.
KEGGtdn:Suden_1445.
PATRIC23771528. VBISulDen68967_1499.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0624.
HOGENOMHOG000243770.
KOK01439.
OMAWDAPRLE.
ProtClustDBPRK13009.

Enzyme and pathway databases

BioCycSDEN326298:GH9P-1504-MONOMER.
UniPathwayUPA00034; UER00021.

Family and domain databases

HAMAPMF_01690. DapE.
InterProIPR001261. ArgE/DapE_CS.
IPR005941. DapE_proteobac.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMSSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMsTIGR01246. dapE_proteo. 1 hit.
PROSITEPS00758. ARGE_DAPE_CPG2_1. False negative.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPE_SULDN
AccessionPrimary (citable) accession number: Q30QK9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: December 6, 2005
Last modified: May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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