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Q304B9 (NCASE_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Neutral ceramidase
Short name=N-CDase
Short name=NCDase
EC=3.5.1.23
Alternative name(s):
Acylsphingosine deacylase
N-acylsphingosine amidohydrolase
Gene names
Ordered Locus Names:At2g38010
ORF Names:T8P21.8
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.

Catalytic activity

N-acylsphingosine + H2O = a carboxylate + sphingosine.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the neutral ceramidase family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processsphingolipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasmodesma

Inferred from direct assay. Source: TAIR

   Molecular functionceramidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q304B9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 757732Neutral ceramidase
PRO_0000247110

Sites

Active site3301Nucleophile By similarity

Amino acid modifications

Glycosylation3111N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation6571N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 6586793BC42B1E42

FASTA75783,264
        10         20         30         40         50         60 
MAVSLPLFQF ILFLLLLLLS RTVYAYLIGV GSYDITGPAA DVNMMGYANS DQIASGIHFR 

        70         80         90        100        110        120 
LRARAFIVAE PQGNRVVFVN LDACMASQIV TIKVLERLKA RYGELYTEKN VAISGIHTHA 

       130        140        150        160        170        180 
GPGGYLQYVT YIVTSLGFVR QSFDVVVNGI EQSIVQAHES LRPGSAFVNK GDLLDAGVNR 

       190        200        210        220        230        240 
SPSSYLNNPA AERSKYKYDV DKEMTLVKFV DSQLGPTGSF NWFATHGTSM SRTNSLISGD 

       250        260        270        280        290        300 
NKGAAARFME DWFENGQKNS VSSRNIPRRV STIVSDFSRN RDRLLDIAAT YKSSRGHSVD 

       310        320        330        340        350        360 
KSLDVKTRVR NGSKRKFVSA FCQSNCGDVS PNTLGTFCID TGLPCDFNHS TCNGQNELCY 

       370        380        390        400        410        420 
GRGPGYPDEF ESTRIIGEKQ FKMAVELFNK ATEKLQGKIG YQHAYLDFSN LDVTVPKAGG 

       430        440        450        460        470        480 
GSETVKTCPA AMGFGFAAGT TDGPGAFDFK QGDDQGNVFW RLVRNVLRTP GPEQVQCQKP 

       490        500        510        520        530        540 
KPILLDTGEM KEPYDWAPSI LPIQILRIGQ LVILSVPGEF TTMAGRRLRD AIKSFLISSD 

       550        560        570        580        590        600 
PKEFSNNMHV VIAGLTNTYS QYIATFEEYE VQRYEGASTL YGRHTLTAYI QEFKKLATAL 

       610        620        630        640        650        660 
VNGLTLPRGP QPPDLLDKQI SLLSPVVVDS TPLGVKFGDV KADVPPKSTF RRGQQVNATF 

       670        680        690        700        710        720 
WSGCPRNDLM TEGSFAVVET LREGGKWAPV YDDDDFSLKF KWSRPAKLSS ESQATIEWRV 

       730        740        750 
PESAVAGVYR IRHYGASKSL FGSISSFSGS SSAFVVV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002685 Genomic DNA. Translation: AEC09477.1.
IPIIPI00521783.
PIRH84799.
RefSeqNP_181337.2. NM_129358.3.
UniGeneAt.21726.
At.69624.

3D structure databases

ProteinModelPortalQ304B9.
SMRQ304B9. Positions 24-757.
ModBaseSearch...

Proteomic databases

PRIDEQ304B9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G38010.1; AT2G38010.1; AT2G38010.
GeneID818379.
GenomeReviewsGene locus AT2G38010 in contig CT485783_GR.
KEGGath:AT2G38010.
NMPDRfig|3702.1.peg.10907.

Organism-specific databases

TAIRAt2g38010.

Phylogenomic databases

eggNOGKOG2232.
GeneTreeEPGT00050000018564.
HOGENOMHBG315824.
InParanoidQ304B9.
PhylomeDBQ304B9.
ProtClustDBCLSN2682562.

Gene expression databases

GenevestigatorQ304B9.
GermOnlineAT2G38010. Arabidopsis thaliana.

Family and domain databases

InterProIPR006823. Ceramidase_alk.
[Graphical view]
KOK12349.
PANTHERPTHR12670. Ceramidase_alk. 1 hit.
PfamPF04734. Ceramidase_alk. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCASE_ARATH
AccessionPrimary (citable) accession number: Q304B9
Secondary accession number(s): Q3EBK8, Q7XJQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: December 6, 2005
Last modified: December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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