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Protein

CCA-adding enzyme

Gene

cca

Organism
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28ATP or CTP; via amide nitrogenUniRule annotation1
Binding sitei31ATP or CTPUniRule annotation1
Metal bindingi41MagnesiumUniRule annotation1
Metal bindingi43MagnesiumUniRule annotation1
Binding sitei112ATP or CTPUniRule annotation1
Binding sitei155ATP or CTPUniRule annotation1
Binding sitei158ATP or CTPUniRule annotation1
Binding sitei161ATP or CTPUniRule annotation1
Binding sitei164ATP or CTPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:SAB1321c
OrganismiStaphylococcus aureus (strain bovine RF122 / ET3-1)
Taxonomic identifieri273036 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000543341 – 400CCA-adding enzymeAdd BLAST400

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2YY49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000253346.
KOiK00974.
OMAiERITVEW.

Family and domain databases

CDDicd05398. NT_ClassII-CCAase. 1 hit.
HAMAPiMF_01263. CCA_bact_type3. 1 hit.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YY49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKSLFEQAR PILEQIQDNG FEAYYVGGSV RDYVMGRNIH DIDITTSATP
60 70 80 90 100
DEIESIFSHT IPVGKEHGTI NVVFNNENYE VTTFRAEEDY VDHRRPSGVT
110 120 130 140 150
FVRDLYEDLQ RRDFTMNAIA MDTAYKLYDY FDGQQDINNR IIRTVGIAAE
160 170 180 190 200
RFQEDALRMI RCLRFQSQLS FEIATETFEA MRTQMADIKF LSIERIVIEL
210 220 230 240 250
TKLMRGINVE KSFNHLKSLK AFNYMPYFEH LDMNQINVTE PIDLELLIAI
260 270 280 290 300
VSVKFDINYS LKPLKLSNRQ VKDINQYIQI MNALPSIFTK EQLKIFVYDY
310 320 330 340 350
DTNLIKNIMV AADVLKANDI QGHEPLIVNL QTIDETLHHL PMHNRKDMMV
360 370 380 390 400
NGGVLMAHLN AKSGPWLKDV LRQIEIAIVT GKVSNEETEI LKWVDNHVKI
Length:400
Mass (Da):46,393
Last modified:December 20, 2005 - v1
Checksum:i36B5D01741BFD6E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ938182 Genomic DNA. Translation: CAI81010.1.
RefSeqiWP_000361551.1. NC_007622.1.

Genome annotation databases

EnsemblBacteriaiCAI81010; CAI81010; SAB1321c.
KEGGisab:SAB1321c.
PATRICi19523523. VBIStaAur92441_1410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ938182 Genomic DNA. Translation: CAI81010.1.
RefSeqiWP_000361551.1. NC_007622.1.

3D structure databases

ProteinModelPortaliQ2YY49.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI81010; CAI81010; SAB1321c.
KEGGisab:SAB1321c.
PATRICi19523523. VBIStaAur92441_1410.

Phylogenomic databases

HOGENOMiHOG000253346.
KOiK00974.
OMAiERITVEW.

Family and domain databases

CDDicd05398. NT_ClassII-CCAase. 1 hit.
HAMAPiMF_01263. CCA_bact_type3. 1 hit.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCCA_STAAB
AccessioniPrimary (citable) accession number: Q2YY49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.