Q2YY41 (Q2YY41_STAAB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Trimethoprim-sensitive dihydrofolate reductase EMBL CAI80970.1
EC=1.5.1.3 EMBL CAI80970.1

Gene names

Name:	dfrB EMBL CAI80970.1
Ordered Locus Names:	SAB1281c

Organism

Staphylococcus aureus (strain bovine RF122 / ET3-1) [Complete proteome] [HAMAP]

Taxonomic identifier

273036 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the dihydrofolate reductase family. RuleBase RU004474

Ontologies

Keywords
Ligand	NADP PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF Nucleotide-binding PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF
Molecular function	Oxidoreductase EMBL CAI80970.1
Technical term	3D-structure PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	19 – 20	2	NADP PDB 3F0X PDB 3FQ0 PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF
	Nucleotide binding	45 – 47	3	NADP PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF
	Nucleotide binding	63 – 65	3	NADP PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF
	Nucleotide binding	95 – 101	7	NADP PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF
Sites
	Binding site	8	1	NADP; via amide nitrogen and carbonyl oxygen PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ
	Binding site	15	1	NADP; via carbonyl oxygen PDB 3F0B PDB 3F0Q PDB 3F0S PDB 3F0U PDB 3F0V PDB 3F0X PDB 3FQ0 PDB 3FQC PDB 3FQO PDB 3FQV PDB 3FQZ PDB 3FQF
	Binding site	122	1	NADP PDB 3FQO

Sequences

	Sequence		Length	Mass (Da)
	Q2YY41 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 811898409FEAFAAB	FASTA	159	18,251
	10 20 30 40 50 60 MTLSILVAHD LQRVIGFENQ LPWHLPNDLK HVKKLSTGHT LVMGRKTFES IGKPLPNRRN 70 80 90 100 110 120 VVLTSDTSFN VEGVDVIHSI EDIYQLPGHV FIFGGQTLFE EMIDKVDDMY ITVIEGKFRG 130 140 150 DTFFPPYTFE DWEVASSVEG KLDEKNTIPH TFLHLIRKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular correlates of host specialization in Staphylococcus aureus." Herron-Olson L., Fitzgerald J.R., Musser J.M., Kapur V. PLoS ONE 2:E1120-E1120(2007) [PubMed: 17971880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Crystal structures of wild-type and mutant methicillin-resistant Staphylococcus aureus dihydrofolate reductase reveal an alternate conformation of NADPH that may be linked to trimethoprim resistance." Frey K.M., Liu J., Lombardo M.N., Bolstad D.B., Wright D.L., Anderson A.C. J. Mol. Biol. 387:1298-1308(2009) [PubMed: 19249312] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 2-158 IN COMPLEX WITH NADP.
[3]	"Predicting resistance mutations using protein design algorithms." Frey K.M., Georgiev I., Donald B.R., Anderson A.C. Proc. Natl. Acad. Sci. U.S.A. 107:13707-13712(2010) [PubMed: 20643959] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 2-158 IN COMPLEX WITH NADP.
[4]	"Crystallographic Complexes of Wildtype and Mutant MRSA DHFR Reveal Interactions for Lead Design." Frey K.M., Liu J., Lombardo M.N., Bolstad D.B., Smith A.E., Priestley N.D., Wright D.L., Anderson A.C. Submitted (OCT-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 2-158 IN COMPLEX WITH NADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ938182 Genomic DNA. Translation: CAI80970.1.

RefSeq

YP_416758.1. NC_007622.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3F0B	X-ray	2.10	X	2-158	[»]
3F0Q	X-ray	2.08	X	2-158	[»]
3F0S	X-ray	2.70	X	2-158	[»]
3F0U	X-ray	1.60	X	2-158	[»]
3F0V	X-ray	2.35	X	2-158	[»]
3F0X	X-ray	2.60	X	2-158	[»]
3FQ0	X-ray	1.69	A	2-158	[»]
3FQC	X-ray	2.35	A/B	2-158	[»]
3FQF	X-ray	1.77	A	2-158	[»]
3FQO	X-ray	2.09	A	2-158	[»]
3FQV	X-ray	1.85	A	2-158	[»]
3FQZ	X-ray	1.72	A	2-158	[»]

ProteinModelPortal

Q2YY41.

ModBase

Search...

Protein-protein interaction databases

STRING

Q2YY41.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTAT00000011791; EBSTAP00000011448; EBSTAG00000011790.

GeneID

3794481.

GenomeReviews

Gene locus SAB1281c in contig AJ938182_GR.

KEGG

sab:SAB1281c.

PATRIC

19523435. VBIStaAur92441_1366.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0262.

GeneTree

EBGT00050000024106.

HOGENOM

HBG665708.

OMA

QDEEGVI.

PhylomeDB

Q2YY41.

ProtClustDB

CLSK885344.

Enzyme and pathway databases

BioCyc

SAUR273036:SAB1281C-MONOMER.

Family and domain databases

InterPro

IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]

Gene3D

G3DSA:3.40.430.10. G3DSA:3.40.430.10. 1 hit.

KO

K00287.

PANTHER

PTHR11549:SF1. DHFR. 1 hit.

Pfam

PF00186. DHFR_1. 1 hit.
[Graphical view]

PIRSF

PIRSF000194. DHFR. 1 hit.

PRINTS

PR00070. DHFR.

SUPFAM

SSF53597. SSF53597. 1 hit.

PROSITE

PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q2YY41_STAAB

Accession

Primary (citable) accession number: Q2YY41

Entry history

Integrated into UniProtKB/TrEMBL:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)