Q2YY04 (ARLS_STAAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal transduction histidine-protein kinase ArlS
EC=2.7.13.3

Gene names

Name:	arlS
Ordered Locus Names:	SAB1270c

Organism

Staphylococcus aureus (strain bovine RF122 / ET3-1) [Complete proteome] [HAMAP]

Taxonomic identifier

273036 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to ArlR By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system Virulence
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Signal transduction histidine-protein kinase ArlS		PRO_0000293447
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	156 – 176	21	Helical; Potential
	Domain	178 – 231	54	HAMP
	Domain	239 – 451	213	Histidine kinase
Amino acid modifications
	Modified residue	242	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YY04 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 955C0BCC364E9226	FASTA	451	52,367
	10 20 30 40 50 60 MTKRKLHNNW IIVTTMITFV TIFLFCLIII FFLKDTLHNS ELDDAERSSS DINNLFHSKP 70 80 90 100 110 120 VKDISALDLN ASLGNFQEII IYDEHNNKLF ETSNDNTVRV EPGYEHRYFD RVIKKRYKGI 130 140 150 160 170 180 DYLIIKEPIT TQDFKGYSLL IHSLENYDNI VKSLYIIALA FGVIATIITA TISYVFSTQI 190 200 210 220 230 240 TKPLVSLSNK MIEIRRDGFQ NKLQLNTNYE EIDNLANTFN EMMSQIEESF NQQRQFVEDA 250 260 270 280 290 300 SHELRTPLQI IQGHLNLIQR WGKKDPAVLE ESLNISIEEM NRIIKLVEEL LELTKGDVND 310 320 330 340 350 360 ISSEAQTVHI NDEIRSRIHS LKQLHPDYQF DTDLTSKNLE IKMKPHQFEQ LFLIFIDNAI 370 380 390 400 410 420 KYDVKNKKIK VKTRLKNKQK IIEITDHGIG IPEEDQDFIF DRFYRVDKSR SRSQGGNGLG 430 440 450 LSIAQKIIQL NGGSIKIKSE INKGTTFKII F

References

[1]	"Molecular correlates of host specialization in Staphylococcus aureus." Herron-Olson L., Fitzgerald J.R., Musser J.M., Kapur V. PLoS ONE 2:E1120-E1120(2007) [PubMed: 17971880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: bovine RF122 / ET3-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ938182 Genomic DNA. Translation: CAI80959.1.
RefSeq	YP_416747.1. NC_007622.1.
3D structure databases
HSSP	HSSP built from PDB template 2C2A based on UniProtKB Q9WZV7.
ProteinModelPortal	Q2YY04.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YY04.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000012888; EBSTAP00000012545; EBSTAG00000012887.
GeneID	3794949.
GenomeReviews	Gene locus SAB1270c in contig AJ938182_GR.
KEGG	sab:SAB1270c.
NMPDR	fig|273036.3.peg.830.
PATRIC	19523411. VBIStaAur92441_1354.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0642.
GeneTree	EBGT00050000023863.
HOGENOM	HBG694527.
OMA	FSSQITK.
PhylomeDB	Q2YY04.
ProtClustDB	CLSK885336.
Enzyme and pathway databases
BioCyc	SAUR273036:SAB1270C-MONOMER.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLS_STAAB

Accession

Primary (citable) accession number: Q2YY04

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program