Q2YV76 (ISPD1_STAAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
MEP cytidylyltransferase 1
Short name=MCT 1

Gene names

Name:	ispD1
Ordered Locus Names:	SAB0190

Organism

Staphylococcus aureus (strain bovine RF122 / ET3-1) [Complete proteome] [HAMAP]

Taxonomic identifier

273036 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108		PRO_0000237824
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YV76 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: AA66EB71255E700C	FASTA	238	26,574
	10 20 30 40 50 60 MIYAGILAGG IGSRMGNVPL PKQFLDIDNK PILIHTIEKF ILVSEFNEII IATPAQWISH 70 80 90 100 110 120 TQDILKKYNI TDQRVKVVAG GTDRNETIMN IIDHIRNVNG INNNDVIVTH DAVRPFLTQR 130 140 150 160 170 180 IIKENIEVAA KYGAVDTVIE AIDTIVMSKD KQNIHSIPVR NEMYQGQTPQ SFNIKLLQDS 190 200 210 220 230 YRALSSEQKE ILSDACKIIV ESGHAVKLVR GELYNIKVTT PYDLKVANAI IQGDIADD

References

[1]	"Molecular correlates of host specialization in Staphylococcus aureus." Herron-Olson L., Fitzgerald J.R., Musser J.M., Kapur V. PLoS ONE 2:E1120-E1120(2007) [PubMed: 17971880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: bovine RF122 / ET3-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ938182 Genomic DNA. Translation: CAI79878.1.
RefSeq	YP_415698.1. NC_007622.1.
3D structure databases
ProteinModelPortal	Q2YV76.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YV76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000012421; EBSTAP00000012078; EBSTAG00000012420.
GeneID	3793574.
GenomeReviews	Gene locus SAB0190 in contig AJ938182_GR.
KEGG	sab:SAB0190.
NMPDR	fig|273036.3.peg.408.
PATRIC	19521093. VBIStaAur92441_0215.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
GeneTree	EBGT00050000024510.
HOGENOM	HBG672839.
OMA	GELFNIK.
PhylomeDB	Q2YV76.
ProtClustDB	PRK13385.
Enzyme and pathway databases
BioCyc	SAUR273036:SAB0190-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD1_STAAB

Accession

Primary (citable) accession number: Q2YV76

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program