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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1

Gene

ispD1

Organism
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (ispD1)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerUniRule annotation
Sitei22 – 221Transition state stabilizerUniRule annotation
Sitei160 – 1601Positions MEP for the nucleophilic attackUniRule annotation
Sitei217 – 2171Positions MEP for the nucleophilic attackUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

BioCyciSAUR273036:GJVS-192-MONOMER.
UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1UniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1UniRule annotation
MEP cytidylyltransferase 1UniRule annotation
Short name:
MCT 1UniRule annotation
Gene namesi
Name:ispD1UniRule annotation
Ordered Locus Names:SAB0190
OrganismiStaphylococcus aureus (strain bovine RF122 / ET3-1)
Taxonomic identifieri273036 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2382382-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1PRO_0000237824Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ2YV76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218563.
KOiK00991.
OMAiGACDTVI.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YV76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYAGILAGG IGSRMGNVPL PKQFLDIDNK PILIHTIEKF ILVSEFNEII
60 70 80 90 100
IATPAQWISH TQDILKKYNI TDQRVKVVAG GTDRNETIMN IIDHIRNVNG
110 120 130 140 150
INNNDVIVTH DAVRPFLTQR IIKENIEVAA KYGAVDTVIE AIDTIVMSKD
160 170 180 190 200
KQNIHSIPVR NEMYQGQTPQ SFNIKLLQDS YRALSSEQKE ILSDACKIIV
210 220 230
ESGHAVKLVR GELYNIKVTT PYDLKVANAI IQGDIADD
Length:238
Mass (Da):26,574
Last modified:December 20, 2005 - v1
Checksum:iAA66EB71255E700C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ938182 Genomic DNA. Translation: CAI79878.1.
RefSeqiWP_000638478.1. NC_007622.1.

Genome annotation databases

EnsemblBacteriaiCAI79878; CAI79878; SAB0190.
KEGGisab:SAB0190.
PATRICi19521093. VBIStaAur92441_0215.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ938182 Genomic DNA. Translation: CAI79878.1.
RefSeqiWP_000638478.1. NC_007622.1.

3D structure databases

ProteinModelPortaliQ2YV76.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI79878; CAI79878; SAB0190.
KEGGisab:SAB0190.
PATRICi19521093. VBIStaAur92441_0215.

Phylogenomic databases

HOGENOMiHOG000218563.
KOiK00991.
OMAiGACDTVI.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.
BioCyciSAUR273036:GJVS-192-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISPD1_STAAB
AccessioniPrimary (citable) accession number: Q2YV76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.