Reviewed, UniProtKB/Swiss-Prot Q2YV73 (ISPD2_STAAB)

Names and origin

Protein names

Recommended name:
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2
    EC=2.7.7.60
Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2
    MEP cytidylyltransferase 2
      Short name=MCT 2

Gene names

Name:	ispD2
Ordered Locus Names:	SAB0194

Organism

Staphylococcus aureus (strain bovine RF122 / ET3-1) [Complete proteome] [HAMAP]

Taxonomic identifier

273036 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the ispD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108		PRO_0000237823
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YV73-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: EB95DE02272E7A02	FASTA	238	26,658
	10 20 30 40 50 60 MKYAGILAGG IGSRMGNVPL PKQFLDLDNK PILIHTLEKF ILINDFEKII IATPQQWMTH 70 80 90 100 110 120 TKDTLRKFKI SDERIEVIQG GSDRNDTIMN IVKHIESTNG INDDDVIVTH DAVRPFLTHR 130 140 150 160 170 180 IIKENIQAAL EYGAVDTVID AIDTIVTSKD DQTIDAIPVR NEMYQGQTPQ SFNINLLKES 190 200 210 220 230 YAQLSDEQKS ILSDACKIIV ETNKPVRLVK GELYNIKVTT PYDLKVANAI IRGGIADD

References

[1]	"Molecular correlates of host specialization in Staphylococcus aureus." Herron-Olson L., Fitzgerald J.R., Musser J.M., Kapur V. PLoS ONE 2:E1120-E1120(2007) [PubMed: 17971880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ938182 Genomic DNA. Translation: CAI79882.1.
RefSeq	YP_415701.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YV73.
Genome annotation databases
GeneID	3793578.
GenomeReviews	Gene locus SAB0194 in contig AJ938182_GR.
KEGG	sab:SAB0194.
NMPDR	fig|273036.3.peg.411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YV73.
OMA	REQQDFW.
Enzyme and pathway databases
BioCyc	SAUR273036:SAB0194-MON.
Family and domain databases
HAMAP	MF_00108. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD2_STAAB

Accession

Primary (citable) accession number: Q2YV73

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 20, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)