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Q2YV52

- PFLA_STAAB

UniProt

Q2YV52 - PFLA_STAAB

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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi33 – 331Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi36 – 361Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR273036:GJVS-167-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Ordered Locus Names:SAB0165
OrganismiStaphylococcus aureus (strain bovine RF122 / ET3-1)
Taxonomic identifieri273036 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001927: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Pyruvate formate-lyase-activating enzymePRO_0000271710Add
BLAST

Proteomic databases

PRIDEiQ2YV52.

Interactioni

Protein-protein interaction databases

STRINGi273036.SAB0165.

Structurei

3D structure databases

ProteinModelPortaliQ2YV52.
SMRiQ2YV52. Positions 71-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiVAMSNSE.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YV52-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKGHLHSVE SLGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKISEPSRE
60 70 80 90 100
VTVDEMVNEI LPYKPYFDAS GGGVTVSGGE PLLQMPFLEK LFAELKENGV
110 120 130 140 150
HTCLDTSAGC ANDTKAFQRH FEELQKHTDL ILLDIKHIDN DKHIRLTGKP
160 170 180 190 200
NTHILNFARK LSDMKQPVWI RHVLVPGYSD DKDDLIKLGE FINSLDNVEK
210 220 230 240 250
FEILPYHQLG VHKWKTLGIA YELEDVEAPD DEAVKAAYRY VNFKGKIPVE

L
Length:251
Mass (Da):28,499
Last modified:December 20, 2005 - v1
Checksum:i892A603E273F6C89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ938182 Genomic DNA. Translation: CAI79853.1.
RefSeqiYP_415675.1. NC_007622.1.

Genome annotation databases

EnsemblBacteriaiCAI79853; CAI79853; SAB0165.
GeneIDi3794307.
KEGGisab:SAB0165.
PATRICi19521037. VBIStaAur92441_0187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ938182 Genomic DNA. Translation: CAI79853.1 .
RefSeqi YP_415675.1. NC_007622.1.

3D structure databases

ProteinModelPortali Q2YV52.
SMRi Q2YV52. Positions 71-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273036.SAB0165.

Proteomic databases

PRIDEi Q2YV52.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI79853 ; CAI79853 ; SAB0165 .
GeneIDi 3794307.
KEGGi sab:SAB0165.
PATRICi 19521037. VBIStaAur92441_0187.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011458.
KOi K04069.
OMAi VAMSNSE.
OrthoDBi EOG64FKHC.

Enzyme and pathway databases

BioCyci SAUR273036:GJVS-167-MONOMER.

Family and domain databases

InterProi IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular correlates of host specialization in Staphylococcus aureus."
    Herron-Olson L., Fitzgerald J.R., Musser J.M., Kapur V.
    PLoS ONE 2:E1120-E1120(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: bovine RF122 / ET3-1.

Entry informationi

Entry nameiPFLA_STAAB
AccessioniPrimary (citable) accession number: Q2YV52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3