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Protein

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Gene

mtnN

Organism
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine.UniRule annotation
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Proton acceptorUniRule annotation1
Binding sitei77Substrate; via amide nitrogenUniRule annotation1
Binding sitei151Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei196Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BioCyciSAUR273036:G1G1D-1615-MONOMER
UniPathwayiUPA00904; UER00871

Names & Taxonomyi

Protein namesi
Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseUniRule annotation (EC:3.2.2.9UniRule annotation)
Short name:
MTA/SAH nucleosidaseUniRule annotation
Short name:
MTANUniRule annotation
Alternative name(s):
5'-methylthioadenosine nucleosidaseUniRule annotation
Short name:
MTA nucleosidaseUniRule annotation
S-adenosylhomocysteine nucleosidaseUniRule annotation
Short name:
AdoHcy nucleosidaseUniRule annotation
Short name:
SAH nucleosidaseUniRule annotation
Short name:
SRH nucleosidaseUniRule annotation
Gene namesi
Name:mtnNUniRule annotation
Ordered Locus Names:SAB1471c
OrganismiStaphylococcus aureus (strain bovine RF122 / ET3-1)
Taxonomic identifieri273036 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003593621 – 2285'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseAdd BLAST228

Structurei

3D structure databases

ProteinModelPortaliQ2YT29
SMRiQ2YT29
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 173Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000259346
KOiK01243
OMAiDQFVHSK

Family and domain databases

HAMAPiMF_01684 Salvage_MtnN, 1 hit
InterProiView protein in InterPro
IPR010049 MTA_SAH_Nsdase
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01704 MTA/SAH-Nsdase, 1 hit

Sequencei

Sequence statusi: Complete.

Q2YT29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGIIGAMEE EVTILKNKLT QLSEISVAHV KFYTGILKDR EVVITQSGIG
60 70 80 90 100
KVNAAISTTL LINKFKPDII INTGSAGALD ESLNVGDVLI SDDVKYHDAD
110 120 130 140 150
ATAFGYEYGQ IPQMPVAFQS SKPLIEKVSQ VVQQQQLTAK VGLIVSGDSF
160 170 180 190 200
IGSVEQRQKI KKAFPNAMAV EMEATAIAQT CYQFNVPFVV VRAVSDLANG
210 220
EAEISFEAFL EKAAVSSSQT VEALVSQL
Length:228
Mass (Da):24,530
Last modified:December 20, 2005 - v1
Checksum:i609C1603FE88A1EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ938182 Genomic DNA Translation: CAI81160.1
RefSeqiWP_000579266.1, NC_007622.1

Genome annotation databases

EnsemblBacteriaiCAI81160; CAI81160; SAB1471c
KEGGisab:SAB1471c

Similar proteinsi

Entry informationi

Entry nameiMTNN_STAAB
AccessioniPrimary (citable) accession number: Q2YT29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: December 20, 2005
Last modified: February 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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