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Q2YS10 (HEM6_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oxygen-dependent coproporphyrinogen-III oxidase

Short name=CPO
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3
Gene names
Name:hemF
Ordered Locus Names:BAB1_1566
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX By similarity. HAMAP-Rule MF_00333

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP-Rule MF_00333

Cofactor

Divalent cations By similarity. HAMAP-Rule MF_00333

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP-Rule MF_00333

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00333

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00333.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   Cellular componentCytoplasm
   LigandMetal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Oxygen-dependent coproporphyrinogen-III oxidase HAMAP-Rule MF_00333
PRO_1000119787

Regions

Region132 – 1343Substrate binding By similarity
Region268 – 30336Important for dimerization By similarity
Region286 – 2883Substrate binding By similarity

Sites

Active site1301Proton donor By similarity
Metal binding1201Divalent cation By similarity
Metal binding1301Divalent cation By similarity
Metal binding1681Divalent cation By similarity
Metal binding1991Divalent cation By similarity
Binding site1161Substrate By similarity
Site1991Important for dimerization By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YS10 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 65C4C1B754D13CE0

FASTA30335,111
        10         20         30         40         50         60 
MKREDIPAII PADIEEKKKA AQSWFEELRD RICASYEQLE DELQGPLSDR EPGRFVRTPW 

        70         80         90        100        110        120 
QKDDGNGGGV MSIMHGRVFE KVGVHVSTVH GEFSPEFRKQ IPGAEEDPRY WASGISLIAH 

       130        140        150        160        170        180 
PQNPNVPAVH MNTRMIVTTR QWFAGGADLT PVLDRRRTQE DPDTLAFHKA FRFICEKHKD 

       190        200        210        220        230        240 
IVDYQRLKEW CDEYFFLPHR DEPRGIGGIF YDWLHSPEEK GGWDSDFAFT RDVGRGFSVV 

       250        260        270        280        290        300 
YPHLVRQNFN KDWTEADRDE QLIRRGRYVE FNLLYDRGTI FGLKTGGNMN AILSSMPPVV 


KWP 

« Hide

References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040264 Genomic DNA. Translation: CAJ11522.1.
RefSeqYP_414932.1. NC_007618.1.

3D structure databases

ProteinModelPortalQ2YS10.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB1_1566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ11522; CAJ11522; BAB1_1566.
GeneID3788746.
KEGGbmf:BAB1_1566.
PATRIC17846127. VBIBruMel86222_1625.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0408.
HOGENOMHOG000262768.
KOK00228.
OMAVKAYLLD.
OrthoDBEOG67T5GJ.
ProtClustDBPRK05330.

Enzyme and pathway databases

BioCycBABO359391:GKDV-1599-MONOMER.
BMEL359391:GJOQ-1599-MONOMER.
UniPathwayUPA00251; UER00322.

Family and domain databases

Gene3D3.40.1500.10. 1 hit.
HAMAPMF_00333. Coprogen_oxidas.
InterProIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERPTHR10755. PTHR10755. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
SUPFAMSSF102886. SSF102886. 1 hit.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM6_BRUA2
AccessionPrimary (citable) accession number: Q2YS10
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 7, 2006
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names