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Q2YRW5 (QUEF_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified August 10, 2010. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
Customize displayNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase

EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
PreQ(0) reductase
NADPH-dependent nitrile oxidoreductase
Gene names
Name:queF
Ordered Locus Names:BAB1_1206
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length155 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818

Subcellular location

Cytoplasm Probable HAMAP MF_00818.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

queuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: InterPro

queuine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 155155NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818
PRO_0000247679

Sequences

Sequence LengthMass (Da)Tools
Q2YRW5-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: BEC57D5171BBC051

FASTA15517,402
        10         20         30         40         50         60 
MSENTIYSGL KQLGSHTDIP LTPEEAVLER VANPQEGTPY CVRFTAPEFS SLCPMTGQPD 

        70         80         90        100        110        120 
FAHLVIDYVP GKWLVESKSL KLFLFSFRNH GAFHEDCTVT IGKRLVDLLE PEWLRIGGYW 

       130        140        150 
YPRGGIPIDV FYQTGAAPLN VWIPEQGVAN YRGRG 

« Hide

References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040264 Genomic DNA. Translation: CAJ11162.1.
RefSeqYP_414592.1.

3D structure databases

ProteinModelPortalQ2YRW5.
SMRQ2YRW5. Positions 55-146.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2YRW5.

Genome annotation databases

GeneID3787830.
GenomeReviewsGene locus BAB1_1206 in contig AM040264_GR.
KEGGbmf:BAB1_1206.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHBG294103.
OMAHEDCTVA.
ProtClustDBPRK13258.

Enzyme and pathway databases

BioCycBMEL359391:BAB1_1206-MONOMER.

Family and domain databases

HAMAPMF_00818. QueF_type1.
[Tree]
InterProIPR016856. CN_OxRdtase_NADPH-dep_QueF.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsTIGR03139. QueF-II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_BRUA2
AccessionPrimary (citable) accession number: Q2YRW5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: December 20, 2005
Last modified: August 10, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families