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Protein

Protein RecA

Gene

recA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs (By similarity). Interacts with LexA causing LexA activation and leading to its autocatalytic cleavage (PubMed:16816190). High basal expression of RecA may be important in slowly-dividing Brucella for intracellular survival in its hostile host (PubMed:16816190).UniRule annotation1 Publication
Confers methyl methanesulfonate (MMS) resistance to an E.coli recA deletion mutant (PubMed:8321120). This protein is constitutively activated; its expression in an E.coli recA deletion leads to constitutive expression from a recA reporter gene. Presumably it constitutively activates LexA autocleavage, but no data was published (PubMed:16816190).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 84ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecAUniRule annotation
Alternative name(s):
Recombinase AUniRule annotation
Gene namesi
Name:recAUniRule annotation
Ordered Locus Names:BAB1_1224
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Greatly increased sensitivity to MMS, decreased bacterial load in BALB/c mice, but bacteria still persist 100 days after peritoneal injection (PubMed:8321120). Increased sensitivity to UV light (but considerably less than expected compared to E.coli), hypersensitivity to MMS, slightly increased sensitivity to H2O2. Loss of mitomycin C-induced expression from a recA promoter. 4-fold less survival in mouse macrophages, where the bacteria has to survive an extremely hostile environment (PubMed:16816190).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000478931 – 361Protein RecAAdd BLAST361

Expressioni

Inductioni

Constitutively expressed at a high level with 2-fold further induction by mitomycin C treatment. Positively autoregulated.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ2YRU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000264120.
KOiK03553.
OMAiTRKGAWY.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
TIGRFAMsiTIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YRU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQNSLRLVE DNSVDKTKAL DAALSQIERA FGKGSIMRLG QNDQVVEIET
60 70 80 90 100
VSTGSLSLDI ALGVGGLPKG RIVEIYGPES SGKTTLALHT IAEAQKKGGI
110 120 130 140 150
CAFVDAEHAL DPVYARKLGV DLENLLISQP DTGEQALEIT DTLVRSGAID
160 170 180 190 200
VLVVDSVAAL TPRAEIEGEM GDSLPGLQAR LMSQALRKLT GSISRSNCMV
210 220 230 240 250
IFINQIRMKI GVMFGSPETT TGGNALKFYA SVRLDIRRIG SIKERDEVVG
260 270 280 290 300
NQTRVKVVKN KLAPPFKQVE FDIMYGAGVS KVGELVDLGV KAGVVEKSGA
310 320 330 340 350
WFSYNSQRLG QGRENAKQYL KDNPEVAREI ETTLRQNAGL IAEQFLDDGG
360
PEEDAAGAAE M
Length:361
Mass (Da):38,735
Last modified:December 20, 2005 - v1
Checksum:i6E15766D33E5756E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121D → H in AAA22999 (PubMed:8321120).Curated1
Sequence conflicti131D → I in AAA22999 (PubMed:8321120).Curated1
Sequence conflicti174 – 175LP → H in AAA22999 (PubMed:8321120).Curated2
Sequence conflicti186L → V in AAA22999 (PubMed:8321120).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00679 Genomic DNA. Translation: AAA22999.1.
AM040264 Genomic DNA. Translation: CAJ11180.1.
PIRiI40347.
RefSeqiWP_002964332.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ11180; CAJ11180; BAB1_1224.
GeneIDi3788897.
KEGGibmf:BAB1_1224.
PATRICi17845405. VBIBruMel86222_1272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00679 Genomic DNA. Translation: AAA22999.1.
AM040264 Genomic DNA. Translation: CAJ11180.1.
PIRiI40347.
RefSeqiWP_002964332.1. NZ_KN046823.1.

3D structure databases

ProteinModelPortaliQ2YRU7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ11180; CAJ11180; BAB1_1224.
GeneIDi3788897.
KEGGibmf:BAB1_1224.
PATRICi17845405. VBIBruMel86222_1272.

Phylogenomic databases

HOGENOMiHOG000264120.
KOiK03553.
OMAiTRKGAWY.

Miscellaneous databases

PROiQ2YRU7.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
TIGRFAMsiTIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECA_BRUA2
AccessioniPrimary (citable) accession number: Q2YRU7
Secondary accession number(s): Q04761, Q57CT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.