Q2YRG4 (DUT_BRUA2)
Reviewed,
UniProtKB/Swiss-Prot
Last modified
August 10, 2010.
Version 30.
History...
Names and origin
| Protein names | Recommended name: Deoxyuridine 5'-triphosphate nucleotidohydrolase Short name=dUTPase EC=3.6.1.23 Alternative name(s): dUTP pyrophosphatase | ||||
| Gene names |
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| Organism | Brucella abortus (strain 2308) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 359391 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 157 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116 |
| Catalytic activity | dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116 |
| Cofactor | Magnesium By similarity. HAMAP MF_00116 |
| Pathway | Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116 |
| Sequence similarities | Belongs to the dUTPase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Nucleotide metabolism |
| Ligand | Magnesium Metal-binding |
| Molecular function | Hydrolase |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | dUTP metabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 157 | 157 | Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116 | PRO_0000231401 | |||||
Regions | |||||||||
| Region | 76 – 78 | 3 | Substrate binding By similarity | ||||||
| Region | 93 – 95 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Binding site | 89 | 1 | Substrate By similarity | ||||||
| Binding site | 103 | 1 | Substrate; via amide nitrogen and carbonyl oxygen By similarity | ||||||
Sequences
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References
| [1] | "Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AM040264 Genomic DNA. Translation: CAJ11643.1. | ||||||||||||||||||
| RefSeq | YP_415037.1. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q2YRG4. | ||||||||||||||||||
| SMR | Q2YRG4. Positions 18-139. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| STRING | Q2YRG4. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| GeneID | 3788687. | ||||||||||||||||||
| GenomeReviews | Gene locus BAB1_1687 in contig AM040264_GR. | ||||||||||||||||||
| KEGG | bmf:BAB1_1687. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CMR | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | COG0756. | ||||||||||||||||||
| HOGENOM | HBG436079. | ||||||||||||||||||
| OMA | LDLRACI. | ||||||||||||||||||
| ProtClustDB | PRK00601. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| BioCyc | BMEL359391:BAB1_1687-MONOMER. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| HAMAP | MF_00116. dUTPase_bact. [Tree] | ||||||||||||||||||
| InterPro | IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view] | ||||||||||||||||||
| Pfam | PF00692. dUTPase. 1 hit. [Graphical view] | ||||||||||||||||||
| TIGRFAMs | TIGR00576. dut. 1 hit. | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Entry information
| Entry name | DUT_BRUA2 | ||||||||
| Accession | Primary (citable) accession number: Q2YRG4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Brucella abortus strain 2308 Brucella abortus (strain 2308): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


