Q2YR96 (QUEC_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
7-cyano-7-deazaguanine synthase
EC=6.3.4.20

Alternative name(s):
7-cyano-7-carbaguanine synthase
PreQ(0) synthase
Queuosine biosynthesis protein QueC

Gene names

Name:	queC
Ordered Locus Names:	BAB1_1973

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ0) By similarity. HAMAP-Rule MF_01633
Catalytic activity	7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O. HAMAP-Rule MF_01633
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. HAMAP-Rule MF_01633
Sequence similarities	Belongs to the QueC family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	7-cyano-7-deazaguanine synthase HAMAP-Rule MF_01633		PRO_0000246814
Regions
	Nucleotide binding	7 – 17	11	ATP By similarity
Sites
	Metal binding	185	1	Zinc By similarity
	Metal binding	193	1	Zinc By similarity
	Metal binding	196	1	Zinc By similarity
	Metal binding	199	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YR96 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 0309DDFBB3468597	FASTA	232	25,256
	10 20 30 40 50 60 MKTLVICSGG LDSVSLAHKM AAEHELTGLL SFDYGQRHKK ELDFAQACAK RLGVPHQIID 70 80 90 100 110 120 IRTIGASLTG SALTDDVDVP DGHYAEETMK VTVVPNRNAI MLAIAFGVAA AQKADAVALA 130 140 150 160 170 180 VHGGDHFIYP DCRPGFIEAF QTMQKHALDG YADVKLLAPY VHATKADIVA DGAKYRTPFE 190 200 210 220 230 ATWSCYKGAD RHCGRCGTCV ERREAFHLAG IDDPTSYEDA DFWRATTQKR NA

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ11929.1.
RefSeq	YP_415306.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YR96.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_1973.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ11929; CAJ11929; BAB1_1973.
GeneID	3788434.
KEGG	bmf:BAB1_1973.
PATRIC	17847033. VBIBruMel86222_2055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0603.
HOGENOM	HOG000110564.
KO	K06920.
OMA	HKVIGLN.
ProtClustDB	CLSK864390.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-2025-MONOMER.
UniPathway	UPA00391.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01633. QueC.
InterPro	IPR018317. QueC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF06508. ExsB. 1 hit. [Graphical view]
PIRSF	PIRSF006293. ExsB. 1 hit.
TIGRFAMs	TIGR00364. TIGR00364. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEC_BRUA2

Accession

Primary (citable) accession number: Q2YR96

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program