Reviewed, UniProtKB/Swiss-Prot Q2YR42 (AROK_BRUA2)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BAB1_2030

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Shikimate kinase HAMAP MF_00109		PRO_0000237855
Regions
	Nucleotide binding	33 – 38	6	ATP By similarity
Sites
	Metal binding	37	1	Magnesium By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	101	1	Substrate; via amide nitrogen By similarity
	Binding site	139	1	ATP By similarity
	Binding site	158	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YR42-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: F11D503663A80C62	FASTA	200	22,235
	10 20 30 40 50 60 MSGTNKQTNL HRQTETIRQL LGSKVVVLVG LMGAGKSTIG RKVANMLNLP FKDADTEIET 70 80 90 100 110 120 VSRMTVAELF EAYGEVEFRD LERRVILRLL DDGPMVLATG GGAYMNAETR AAIAEAGISI 130 140 150 160 170 180 WINADLDVLM ERVSRRQNRP LLRNSDPRGV MQRLMDERYP VYALAELHLM TRDEKKEVIA 190 200 AELIEVLAAH LEKEQAASAG

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ11986.1.
RefSeq	YP_415360.1.
3D structure databases
SMR	Q2YR42. Positions 23-173.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YR42.
Genome annotation databases
GeneID	3788623.
GenomeReviews	Gene locus BAB1_2030 in contig AM040264_GR.
KEGG	bmf:BAB1_2030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HBG335815.
OMA	HANVFFV.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_2030-MONOMER.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BRUA2

Accession

Primary (citable) accession number: Q2YR42

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 7, 2006
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)