Q2YR12 (MNMG_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	BAB1_2062

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_1000016557
Regions
	Nucleotide binding	15 – 20	6	FAD By similarity
	Nucleotide binding	274 – 288	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2YR12 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 07B2A517DCF237E0	FASTA	636	69,432
	10 20 30 40 50 60 MSSAEALAFD VIVIGGGHAG CEAASAAARA GARTALVTHR FDTIGVMSCN PAIGGLGKGH 70 80 90 100 110 120 LVREIDALDG LMGRVADRAG IQFRLLNRRK GPAVRGPRTQ ADRKLYRLAM QQMITEQENL 130 140 150 160 170 180 TVVEGGAADL VCDGERISGV TLADGRVLKC GAVVLTTGTF LNGLIHIGEK RFPAGRMGEK 190 200 210 220 230 240 PALGLSERLL SFGFTLGRLK TGTPPRLDGR TIDWQSLDMQ SADEEPVPFS LMTDRITTPQ 250 260 270 280 290 300 IECGITRTTP ETHDIIRANL HRSAMYSGSI EGIGPRYCPS VEDKIVKFGD RDGHQIFLEP 310 320 330 340 350 360 EGLDDDTVYP NGISTSLPED VQLEILKTIP GLEKAVLLQP GYAIEYDFID PRELKRSLET 370 380 390 400 410 420 RKVCGLFLAG QINGTTGYEE AGAQGLLAGL NAARRAAGSE PVILQRTEAY IGVMVDDLTS 430 440 450 460 470 480 RGVSEPYRMF TSRAEFRLSL RADNADQRLT PLADEVGILS EERRKRYLTR ETALSHARMV 490 500 510 520 530 540 TQSLSITPNL AGYYDLRLNQ DGVRRSAYDL LSYPDINLDR LIAIWPELAS IDPVTREALE 550 560 570 580 590 600 IEAQYAVYME RQQSDIAVME REERLLIPSG LDFDAISGLS NELKQKLKQR KPETIAEAQR 610 620 630 VDGMTPAAVA LLIAQIRKFG GRQKLAAETL EGKGAA

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ12018.1.
RefSeq	YP_415390.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YR12.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_2062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ12018; CAJ12018; BAB1_2062.
GeneID	3788723.
KEGG	bmf:BAB1_2062.
PATRIC	17847217. VBIBruMel86222_2144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0445.
HOGENOM	HOG000201059.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-2116-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_BRUA2

Accession

Primary (citable) accession number: Q2YR12

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program