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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ShikimateUniRule annotation
Active sitei73 – 731Proton acceptorUniRule annotation
Binding sitei85 – 851NADPUniRule annotation
Binding sitei94 – 941ShikimateUniRule annotation
Binding sitei109 – 1091ShikimateUniRule annotation
Binding sitei226 – 2261NADP; via carbonyl oxygenUniRule annotation
Binding sitei228 – 2281ShikimateUniRule annotation
Binding sitei249 – 2491NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1385NADPUniRule annotation
Nucleotide bindingi158 – 1636NADPUniRule annotation

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. shikimate 3-dehydrogenase (NADP+) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-HAMAP
  2. chorismate biosynthetic process Source: UniProtKB-UniPathway
  3. shikimate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2124-MONOMER.
BMEL359391:GJOQ-2124-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:BAB1_2070
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Shikimate dehydrogenase (NADP(+))PRO_0000325109Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_2070.

Structurei

3D structure databases

ProteinModelPortaliQ2YR04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 243Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0169.
HOGENOMiHOG000237876.
KOiK00014.
OrthoDBiEOG64R67G.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YR04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDKSMARGR KAFVTGFPIR HSRSPLIHGF WLKELGIDGS YEAVEVKPED
60 70 80 90 100
FSSFAASLAA NGFAGGNVTI PHKEAAYAAA ESLDEAARAI GAVNTLWLEN
110 120 130 140 150
GRLCGGNTDA YGFAANLDAS APGWDKADRA LVLGAGGASR AVVHALLSRG
160 170 180 190 200
VCHVSVVNRT LSRAEELAAH FGARVYAHSW DEAQALVSNA GLIVNTTALG
210 220 230 240 250
MSGHGEGQDF PIDLTCAPKE AVATDIVYVP LRTAFLNKAE KAGLKTVDGL
260 270 280
GMLLHQAVPG FERWFGQRPQ VTQALREHIL ADMAKAGAL
Length:289
Mass (Da):30,520
Last modified:March 18, 2008 - v2
Checksum:iB1B17A56EED18496
GO

Sequence cautioni

The sequence CAJ12026.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ12026.1. Different initiation.
RefSeqiYP_415398.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ12026; CAJ12026; BAB1_2070.
GeneIDi3788516.
KEGGibmf:BAB1_2070.
PATRICi17847235. VBIBruMel86222_2153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ12026.1. Different initiation.
RefSeqiYP_415398.1. NC_007618.1.

3D structure databases

ProteinModelPortaliQ2YR04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi359391.BAB1_2070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ12026; CAJ12026; BAB1_2070.
GeneIDi3788516.
KEGGibmf:BAB1_2070.
PATRICi17847235. VBIBruMel86222_2153.

Phylogenomic databases

eggNOGiCOG0169.
HOGENOMiHOG000237876.
KOiK00014.
OrthoDBiEOG64R67G.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciBABO359391:GKDV-2124-MONOMER.
BMEL359391:GJOQ-2124-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiAROE_BRUA2
AccessioniPrimary (citable) accession number: Q2YR04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: February 4, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.