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Protein

Adenosylhomocysteinase

Gene

ahcY

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.UniRule annotation

Cofactori

NAD+UniRule annotationNote: Binds 1 NAD+ per subunit.UniRule annotation

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (ahcY)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Binding sitei226 – 2261SubstrateUniRule annotation
Binding sitei227 – 2271NADUniRule annotation
Binding sitei279 – 2791NADUniRule annotation
Binding sitei314 – 3141NADUniRule annotation
Binding sitei380 – 3801NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi193 – 1953NADUniRule annotation
Nucleotide bindingi256 – 2616NADUniRule annotation
Nucleotide bindingi335 – 3373NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2153-MONOMER.
BMEL359391:GJOQ-2153-MONOMER.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
AdenosylhomocysteinaseUniRule annotation (EC:3.3.1.1UniRule annotation)
Alternative name(s):
S-adenosyl-L-homocysteine hydrolaseUniRule annotation
Short name:
AdoHcyaseUniRule annotation
Gene namesi
Name:ahcYUniRule annotation
Ordered Locus Names:BAB1_2099
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466AdenosylhomocysteinasePRO_1000024711Add
BLAST

Proteomic databases

PRIDEiQ2YQX8.

Structurei

Secondary structure

1
466
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103Combined sources
Helixi12 – 143Combined sources
Helixi15 – 2612Combined sources
Helixi30 – 3910Combined sources
Turni40 – 423Combined sources
Turni44 – 474Combined sources
Beta strandi49 – 546Combined sources
Helixi58 – 6912Combined sources
Beta strandi73 – 775Combined sources
Helixi86 – 949Combined sources
Beta strandi99 – 1013Combined sources
Helixi107 – 11610Combined sources
Beta strandi127 – 1348Combined sources
Helixi135 – 14713Combined sources
Beta strandi153 – 1553Combined sources
Helixi159 – 17416Combined sources
Helixi178 – 1858Combined sources
Beta strandi188 – 1914Combined sources
Helixi194 – 20613Combined sources
Beta strandi213 – 2153Combined sources
Helixi220 – 2234Combined sources
Helixi226 – 24318Combined sources
Beta strandi251 – 2555Combined sources
Helixi259 – 27012Combined sources
Beta strandi274 – 2785Combined sources
Helixi282 – 2909Combined sources
Helixi298 – 3014Combined sources
Helixi302 – 3043Combined sources
Beta strandi306 – 3105Combined sources
Beta strandi313 – 3186Combined sources
Helixi320 – 3256Combined sources
Beta strandi330 – 3345Combined sources
Beta strandi336 – 3383Combined sources
Turni339 – 3413Combined sources
Helixi345 – 3473Combined sources
Beta strandi350 – 3567Combined sources
Beta strandi359 – 3635Combined sources
Beta strandi369 – 3735Combined sources
Helixi374 – 3763Combined sources
Helixi379 – 3835Combined sources
Helixi389 – 40820Combined sources
Helixi410 – 4123Combined sources
Beta strandi415 – 4184Combined sources
Helixi422 – 43211Combined sources
Helixi434 – 4363Combined sources
Helixi445 – 4517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N58X-ray2.39A/B/C/D8-466[»]
ProteinModelPortaliQ2YQX8.
SMRiQ2YQX8. Positions 5-466.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2YQX8.

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000227986.
KOiK01251.
OMAiKYGCRES.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 2 hits.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YQX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASQDFVVK DISLADWGRK ELDIAETEMP GLMAAREEFG KSQPLKGARI
60 70 80 90 100
SGSLHMTIQT AVLIETLKVL GAEVRWASCN IFSTQDHAAA AIAATGTPVF
110 120 130 140 150
AVKGETLEEY WTYTDQIFQW PDGEPSNMIL DDGGDATMYI LIGARAEAGE
160 170 180 190 200
DVLSNPQSEE EEVLFAQIKK RMAATPGFFT KQRAAIKGVT EETTTGVNRL
210 220 230 240 250
YQLQKKGLLP FPAINVNDSV TKSKFDNKYG CKESLVDGIR RGTDVMMAGK
260 270 280 290 300
VAVVCGYGDV GKGSAQSLAG AGARVKVTEV DPICALQAAM DGFEVVTLDD
310 320 330 340 350
AASTADIVVT TTGNKDVITI DHMRKMKDMC IVGNIGHFDN EIQVAALRNL
360 370 380 390 400
KWTNVKPQVD LIEFPDGKRL ILLSEGRLLN LGNATGHPSF VMSASFTNQV
410 420 430 440 450
LGQIELFTRT DAYKNEVYVL PKHLDEKVAR LHLDKLGAKL TVLSEEQAAY
460
IGVTPQGPFK SEHYRY
Length:466
Mass (Da):50,791
Last modified:February 7, 2006 - v1
Checksum:i6AB3B4B1C2F0BE41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ12055.1.
RefSeqiWP_002965162.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ12055; CAJ12055; BAB1_2099.
GeneIDi3788612.
KEGGibmf:BAB1_2099.
PATRICi17847299. VBIBruMel86222_2185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ12055.1.
RefSeqiWP_002965162.1. NZ_KN046823.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3N58X-ray2.39A/B/C/D8-466[»]
ProteinModelPortaliQ2YQX8.
SMRiQ2YQX8. Positions 5-466.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ2YQX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ12055; CAJ12055; BAB1_2099.
GeneIDi3788612.
KEGGibmf:BAB1_2099.
PATRICi17847299. VBIBruMel86222_2185.

Phylogenomic databases

HOGENOMiHOG000227986.
KOiK01251.
OMAiKYGCRES.

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.
BioCyciBABO359391:GKDV-2153-MONOMER.
BMEL359391:GJOQ-2153-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ2YQX8.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 2 hits.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAHH_BRUA2
AccessioniPrimary (citable) accession number: Q2YQX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.